IL-8 as a urinary biomarker for the detection of bladder cancer

BMC Urology

Research Article

Virginia Urquidi¹  Jeongsoon Kim¹  Steve Goodison¹  Charles J Rosser²  Yunfeng Dai³  Myron Chang³  Edward D Wolfson⁴ 

[1] Cancer Research Institute, MD Anderson Cancer Center Orlando, 32827, Orlando, FL, USA;Cancer Research Institute, MD Anderson Cancer Center Orlando, 32827, Orlando, FL, USA;Department of Pharmacology, The University of Florida, 32610, Gainesville, FL, USA;Section of Urologic Oncology, MD Anderson 391 Cancer Center Orlando, 1400 S. Orange Ave, 32806, Orlando, FL, USA;Department of Biostatistics, The University of Florida, 32611, Gainesville, FL, USA;Department of Pharmacology, The University of Florida, 32610, Gainesville, FL, USA;

关键词: IL-8;    Biomarkers;    Diagnosis;    Bladder cancer;   

DOI  :  10.1186/1471-2490-12-12

 received in 2012-01-20, accepted in 2012-05-04,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundCurrent urine-based assays for bladder cancer (BCa) diagnosis lack accuracy, so the search for improved biomarkers continues. Through genomic and proteomic profiling of urine, we have identified a panel of biomarkers associated with the presence of BCa. In this study, we evaluated the utility of three of these biomarkers, interleukin 8 (IL-8), Matrix metallopeptidase 9 (MMP-9) and Syndecan in the diagnosis of BCa through urinalysis.MethodsVoided urines from 127 subjects, cancer subjects (n = 64), non-cancer subjects (n = 63) were analyzed. The protein concentrations of IL-8, MMP-9, and Syndecan were assessed by enzyme-linked immunosorbent assay (ELISA). Data were also compared to a commercial ELISA-based BCa detection assay (BTA-Trak©) and urinary cytology. We used the area under the curve of a receiver operating characteristic (AUROC) to compare the performance of each biomarker.ResultsUrinary protein concentrations of IL-8, MMP-9 and BTA were significantly elevated in BCa subjects. Of the experimental markers compared to BTA-Trak©, IL-8 was the most prominent marker (AUC; 0.79; 95% confidence interval [CI], 0.72-0.86). Multivariate regression analysis revealed that only IL-8 (OR; 1.51; 95% CI, 1.16-1.97, p = 0.002) was an independent factor for the detection of BCa.ConclusionsThese results suggest that the measurement of IL-8 in voided urinary samples may have utility for urine-based detection of BCa. These findings need to be confirmed in a larger, prospective cohort.

【 授权许可】

Unknown   
© Urquidi et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.