PtrA/NINV, an alkaline/neutral invertase gene of Poncirus trifoliata, confers enhanced tolerance to multiple abiotic stresses by modulating ROS levels and maintaining photosynthetic efficiency

BMC Plant Biology

Research Article

Fei Wang¹  Ji-Hong Liu¹  Ting Peng¹  Bachar Dahro² 

[1] Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Science, Huazhong Agricultural University, 430070, Wuhan, China;Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Science, Huazhong Agricultural University, 430070, Wuhan, China;Department of Horticulture, Faculty of Agriculture, Tishreen University, Lattakia, Syria;

关键词: Poncirus trifoliata;    Abiotic stress;    Alkaline/neutral invertase;    Photosynthetic efficiency;    Sucrose metabolism;    ROS homeostasis;   

DOI  :  10.1186/s12870-016-0761-0

 received in 2015-12-23, accepted in 2016-03-15,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundAlkaline/neutral invertase (A/N-INV), an enzyme that hydrolyzes sucrose irreversibly into glucose and fructose, is essential for normal plant growth,development, and stress tolerance. However, the physiological and/or molecular mechanism underpinning the role of A/N-INV in abiotic stress tolerance is poorly understood.ResultsIn this report, an A/N-INV gene (PtrA/NINV) was isolated from Poncirus trifoliata, a cold-hardy relative of citrus, and functionally characterized. PtrA/NINV expression levels were induced by cold, salt, dehydration, sucrose, and ABA, but decreased by glucose. PtrA/NINV was found to localize in both chloroplasts and mitochondria. Overexpression of PtrA/NINV conferred enhanced tolerance to multiple stresses, including cold, high salinity, and drought, as supported by lower levels of reactive oxygen species (ROS), reduced oxidative damages, decreased water loss rate, and increased photosynthesis efficiency, relative to wild-type (WT). The transgenic plants exhibited higher A/N-INV activity and greater reducing sugar content under normal and stress conditions.ConclusionsPtrA/NINV is an important gene implicated in sucrose decomposition, and plays a positive role in abiotic stress tolerance by promoting osmotic adjustment, ROS detoxification and photosynthesis efficiency. Thus, PtrA/NINV has great potential to be used in transgenic breeding for improvement of stress tolerance.

【 授权许可】

CC BY   
© Dahro et al. 2016