Transcriptome profiling of Kentucky bluegrass (Poa pratensis L.) accessions in response to salt stress

BMC Genomics

Research Article

Keenan L. Amundsen¹  Paul G. Johnson²  Scott E. Warnke³  Joseph G. Robins⁴  B. Shaun Bushman⁴ 

[1] Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE, USA;Department of Plants, Soils, and Climate, Utah State University, Logan, UT, USA;USDA-ARS Floral and Nursery Plants Research Unit, Beltsville, MD, USA;USDA-ARS Forage and Range Research Laboratory, 700 North 1100 East, 84322-6300, Logan, UT, USA;

关键词: Poa pratensis;    Kentucky bluegrass;    Salinity stress;    Transcriptome;    RNA-seq;    Salt tolerance;   

DOI  :  10.1186/s12864-016-2379-x

 received in 2015-09-23, accepted in 2016-01-06,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundKentucky bluegrass (Poa pratensis L.) is a prominent turfgrass in the cool-season regions, but it is sensitive to salt stress. Previously, a relatively salt tolerant Kentucky bluegrass accession was identified that maintained green colour under consistent salt applications. In this study, a transcriptome study between the tolerant (PI 372742) accession and a salt susceptible (PI 368233) accession was conducted, under control and salt treatments, and in shoot and root tissues.ResultsSample replicates grouped tightly by tissue and treatment, and fewer differentially expressed transcripts were detected in the tolerant PI 372742 samples compared to the susceptible PI 368233 samples, and in root tissues compared to shoot tissues. A de novo assembly resulted in 388,764 transcripts, with 36,587 detected as differentially expressed. Approximately 75 % of transcripts had homology based annotations, with several differences in GO terms enriched between the PI 368233 and PI 372742 samples. Gene expression profiling identified salt-responsive gene families that were consistently down-regulated in PI 372742 and unlikely to contribute to salt tolerance in Kentucky bluegrass. Gene expression profiling also identified sets of transcripts relating to transcription factors, ion and water transport genes, and oxidation-reduction process genes with likely roles in salt tolerance.ConclusionsThe transcript assembly represents the first such assembly in the highly polyploidy, facultative apomictic Kentucky bluegrass. The transcripts identified provide genetic information on how this plant responds to and tolerates salt stress in both shoot and root tissues, and can be used for further genetic testing and introgression.

【 授权许可】

CC BY   
© Bushman et al. 2016