Chungsimyeonja-eum inhibits inflammatory responses in RAW 264.7 macrophages and HaCaT keratinocytes

BMC Complementary and Alternative Medicine

Research Article

Hyeun-Kyoo Shin¹  Kim Yeji¹  Sae-Rom Yoo¹  Seong-Eun Jin¹  Chang-Seob Seo¹  Hye-Sun Lim²  Soo-Jin Jeong³ 

[1] K-herb Research Center, Korea Institute of Oriental Medicine, 34054, Daejeon, Republic of Korea;K-herb Research Center, Korea Institute of Oriental Medicine, 34054, Daejeon, Republic of Korea;Division of Allergy and Chronic Respiratory Diseases, Center for Biomedical Sciences, Korea National Institute of Health, 28159, Chungcheongbuk-do, Republic of Korea;Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine, 34054, Daejeon, Republic of Korea;Korean Medicine Life Science, University of Science & Technology, 217 Gajeong-ro Yuseong-gu, 34113, Daejeon, Republic of Korea;

关键词: Chungsimyeonja-eum;    Inflammation;    Cytokine;    Chemokine;    Macrophage;    Keratinocyte;   

DOI  :  10.1186/s12906-015-0902-2

 received in 2015-07-07, accepted in 2015-10-07,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundChungsimyeonja-eum (CSYJE) is an herbal prescription used in traditional Oriental medicine for treating cerebral infarction by reducing ischemic damage. However, the effects of CSYJE on inflammation have not been verified scientifically.MethodsAnti-inflammatory effects of CSYJE was investigated to dertermine the inhibitory effects of CSYJE against inflammation using RAW 264.7 mouse macrophages and HaCaT human keratinocytes. To measure the effects of CSYJE on inflammatory mediators and cytokines/chemokines, we used the following methods: cell viability assay, enzyme-linked immunosorbent assay (ELISA), western blotting, immunocytochemistry. RAW 264.7 cells were pretreated with CSYJE (250, 500, or 1000 μg/mL) for 4 h and treated with lipopolysaccharide (LPS) for additional 20 h. HaCaT cells were stimulated with tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) (TI), and CSYJE (125, 250, or 500 μg/mL) for 24 h.ResultsCSYJE suppressed the production of nitric oxide (NO, IC50 1000 μg/mL), prostaglandin E2 (PGE2, IC50 = 12.1 μg/mL), and interleukin (IL)-6 (IC50 = 248 μg/mL) in LPS-stimulated RAW 264.7 cells. CSYJE suppressed the effects of TI on the production of thymus and activation-regulated chemokine (TARC, IC50 = 330.2 μg/mL), macrophage-derived chemokine (MDC/CCL22, IC50 = 52.5 μg/mL), regulated on activation, normal T-cell expressed and secreted (RANTES/CCL5, IC50 = 372.9 μg/mL), and IL-8 (IC50 = 345.1 μg/mL) in HaCaT cells. CSYJE inhibited TI-stimulated STAT1 phosphorylation in a dose-dependent manner and nuclear translocation at 500 μg/mL in HaCaT cells.ConclusionOur results suggest a possible therapeutic application of CSYJE for treating inflammatory diseases.

【 授权许可】

CC BY   
© Lim et al. 2015