The genome and transcriptome of the pine saprophyte Ophiostoma piceae, and a comparison with the bark beetle-associated pine pathogen Grosmannia clavigera

BMC Genomics

Research Article

Gordon Robertson¹  Shaun Jackman¹  Rod Docking¹  Inanc Birol¹  Sajeet Haridas²  Sepideh Massoumi Alamouti²  Lynette Lim²  Colette Breuil²  Ye Wang²  Jörg Bohlmann³ 

[1] Canada’s Michael Smith Genome Sciences Centre, V5Z 4E6, Vancouver, BC, Canada;Department of Wood Science, University of British Columbia, V6T1Z4, Vancouver, BC, Canada;Michael Smith Laboratories, University of British Columbia, V6T 1Z4, Vancouver, BC, Canada;

关键词: Ophiostoma piceae;    Genome;    Transcriptome;    Wood-staining fungus;    Saprobe;   

DOI  :  10.1186/1471-2164-14-373

 received in 2012-12-05, accepted in 2013-05-10,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundOphiostoma piceae is a wood-staining fungus that grows in the sapwood of conifer logs and lumber. We sequenced its genome and analyzed its transcriptomes under a range of growth conditions. A comparison with the genome and transcriptomes of the mountain pine beetle-associated pathogen Grosmannia clavigera highlights differences between a pathogen that colonizes and kills living pine trees and a saprophyte that colonizes wood and the inner bark of dead trees.ResultsWe assembled a 33 Mbp genome in 45 scaffolds, and predicted approximately 8,884 genes. The genome size and gene content were similar to those of other ascomycetes. Despite having similar ecological niches, O. piceae and G. clavigera showed no large-scale synteny. We identified O. piceae genes involved in the biosynthesis of melanin, which causes wood discoloration and reduces the commercial value of wood products. We also identified genes and pathways involved in growth on simple carbon sources and in sapwood, O. piceae’s natural substrate. Like the pathogen, the saprophyte is able to tolerate terpenes, which are a major class of pine tree defense compounds; unlike the pathogen, it cannot utilize monoterpenes as a carbon source.ConclusionsThis work makes available the second annotated genome of a softwood ophiostomatoid fungus, and suggests that O. piceae’s tolerance to terpenes may be due in part to these chemicals being removed from the cells by an ABC transporter that is highly induced by terpenes. The data generated will provide the research community with resources for work on host-vector-fungus interactions for wood-inhabiting, beetle-associated saprophytes and pathogens.

【 授权许可】

Unknown   
© Haridas et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.