Curcumin supplementation could improve diabetes-induced endothelial dysfunction associated with decreased vascular superoxide production and PKC inhibition

BMC Complementary and Alternative Medicine

Research Article

Naris Thenchaisri¹  Preecha Ruangvejvorachai²  Suthiluk Patumraj³  Sirada Rungseesantivanon⁴ 

[1] Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, 10900, Bangkok, Thailand;Department of Pathology, Faculty of Medicine, Chulalongkorn University, 10330, Bangkok, Thailand;Department of Physiology, Faculty of Medicine, Chulalongkorn University, 10330, Bangkok, Thailand;Inter-department of Physiology, Graduate School, Chulalongkorn University, 10330, Bangkok, Thailand;

关键词: Curcumin;    Superoxide Production;    Diabetes Mellitus Group;    Arteriolar Diameter;    Arteriolar Wall;   

DOI  :  10.1186/1472-6882-10-57

 received in 2009-10-12, accepted in 2010-10-14,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundCurcumin, an Asian spice and food-coloring agent, is known for its anti-oxidant properties. We propose that curcumin can improve diabetes-induced endothelial dysfunction through superoxide reduction.MethodsDiabetes (DM) was induced in rats by streptozotocin (STZ). Daily curcumin oral feeding was started six weeks after the STZ injection. Twelve weeks after STZ injection, mesenteric arteriolar responses were recorded in real time using intravital fluorescence videomicroscopy. Superoxide and vascular protein kinase C (PKC-βII) were examined by hydroethidine and immunofluorescence, respectively.ResultsThe dilatory response to acetylcholine (ACh) significantly decreased in DM arterioles as compared to control arterioles. There was no difference among groups when sodium nitroprusside (SNP) was used. ACh responses were significantly improved by both low and high doses (30 and 300 mg/kg, respectively) of curcumin supplementation. An oxygen radical-sensitive fluorescent probe, hydroethidine, was used to detect intracellular superoxide anion (O2●-) production. O2●- production was markedly increased in DM arterioles, but it was significantly reduced by supplementation of either low or high doses of curcumin. In addition, with a high dose of curcumin, diabetes-induced vascular PKC-βII expression was diminished.ConclusionTherefore, it is suggested that curcumin supplementation could improve diabetes-induced endothelial dysfunction significantly in relation to its potential to decrease superoxide production and PKC inhibition.

【 授权许可】

Unknown   
© Rungseesantivanon et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.