The capability of endophytic fungi for production of hemicellulases and related enzymes

BMC Biotechnology

Research Article

Patrícia dos Santos Costa¹  Priscila da Silva Delabona¹  José Geraldo da Cruz Pradella¹  Ida Chapaval Pimentel²  Vania Aparecida Vicente²  Gabriel Padilla³  Juan Diego Rojas³  Carla Montanari Mergel³  Diogo Robl⁴ 

[1] Brazilian Bioethanol Science and Technology Laboratory – CTBE, Pólo II de Alta Tecnologia, Rua Giuseppe Maximo Scolfaro 10000, CEP 13083-970, Campinas, SP, Brazil;Departament of Basic Pathology, Federal University of Paraná (UFPR), Caixa Postal 19020, CEP 81531-980, Curitiba, PR, Brazil;Institute of Biomedical Sciences, University of São Paulo (USP), Avenida Lineu Prestes 1374 CEP, 05508-900, São Paulo, SP, Brazil;Institute of Biomedical Sciences, University of São Paulo (USP), Avenida Lineu Prestes 1374 CEP, 05508-900, São Paulo, SP, Brazil;Brazilian Bioethanol Science and Technology Laboratory – CTBE, Pólo II de Alta Tecnologia, Rua Giuseppe Maximo Scolfaro 10000, CEP 13083-970, Campinas, SP, Brazil;

关键词: Endophytic fungi;    Xylanase;    Hemicellulases;    Accessory enzymes;   

DOI  :  10.1186/1472-6750-13-94

 received in 2013-05-20, accepted in 2013-09-12,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThere is an imperative necessity for alternative sources of energy able to reduce the world dependence of fossil oil. One of the most successful options is ethanol obtained mainly from sugarcane and corn fermentation. The foremost residue from sugarcane industry is the bagasse, a rich lignocellulosic raw material uses for the production of ethanol second generation (2G). New cellulolytic and hemicellulytic enzymes are needed, in order to optimize the degradation of bagasse and production of ethanol 2G.ResultsThe ability to produce hemicellulases and related enzymes, suitable for lignocellulosic biomass deconstruction, was explored using 110 endophytic fungi and 9 fungi isolated from spoiled books in Brazil. Two initial selections were performed, one employing the esculin gel diffusion assay, and the other by culturing on agar plate media with beechwood xylan and liquor from the hydrothermal pretreatment of sugar cane bagasse. A total of 56 isolates were then grown at 29°C on steam-exploded delignified sugar cane bagasse (DEB) plus soybean bran (SB) (3:1), with measurement of the xylanase, pectinase, β-glucosidase, CMCase, and FPase activities. Twelve strains were selected, and their enzyme extracts were assessed using different substrates. Finally, the best six strains were grown under xylan and pectin, and several glycohydrolases activities were also assessed. These strains were identified morphologically and by sequencing the internal transcribed spacer (ITS) regions and the partial β-tubulin gene (BT2). The best six strains were identified as Aspergillus niger DR02, Trichoderma atroviride DR17 and DR19, Alternaria sp. DR45, Annulohypoxylon stigyum DR47 and Talaromyces wortmannii DR49. These strains produced glycohydrolases with different profiles, and production was highly influenced by the carbon sources in the media.ConclusionsThe selected endophytic fungi Aspergillus niger DR02, Trichoderma atroviride DR17 and DR19, Alternaria sp. DR45, Annulohypoxylon stigyum DR47 and Talaromyces wortmannii DR49 are excellent producers of hydrolytic enzymes to be used as part of blends to decompose sugarcane biomass at industrial level.

【 授权许可】

Unknown   
© Robl et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.