A novel human leiomyoma tissue derived matrix for cell culture studies

BMC Cancer

Research Article

Fumi Suomi¹  Lauri Eklund¹  Ehsanul Hoque Apu²  Meeri Sutinen²  Carolina Cavalcante Bitu²  Pirjo Åström²  Elias Sundquist²  Johanna Korvala²  Tuula Salo³  Saad Ullah Akram⁴  Nilva K. Cervigne⁵  Markku Santala⁶  Kalle Savolainen⁷  Ricardo D. Coletta⁸  Carine Ervolino de Oliveira⁸  Pirjo Juusela⁹  Steffen Ohlmeier^1,10  Adriana Franco Paes Leme^1,11 

[1] Biocenter Oulu, University of Oulu, FI-90014, Oulu, Finland;Faculty of Biochemistry and Molecular Medicine, University of Oulu, FI-90014, Oulu, Finland;Cancer and Translational Medicine Research Unit, Faculty of Medicine, University of Oulu, PO Box 5281, FI-90014, Oulu, Finland;Medical Research Center Oulu, Oulu University Hospital and University of Oulu, FI-90014, Oulu, Finland;Cancer and Translational Medicine Research Unit, Faculty of Medicine, University of Oulu, PO Box 5281, FI-90014, Oulu, Finland;Medical Research Center Oulu, Oulu University Hospital and University of Oulu, FI-90014, Oulu, Finland;Department of Oral and Maxillofacial Diseases, University of Helsinki, FI-00014, Helsinki, Finland;Center for Machine Vision Research, University of Oulu, FI-90014, Oulu, Finland;Biocenter Oulu, University of Oulu, FI-90014, Oulu, Finland;Clinical Department, Faculty of Medicine of Jundiai (FMJ), SP-13202-550, Jundiai, São Paulo, Brazil;Department of Oral Diagnosis, Oral Pathology Division, Piracicaba Dental School, University of Campinas, SP-13414-903, Piracicaba, São Paulo, Brazil;Department of Obstetrics and Gynecology, Oulu University Hospital and University of Oulu, FI-90029, Oulu, Finland;Department of Obstetrics and Gynecology, Tampere University Hospital and University of Tampere, FI-33521, Tampere, Finland;Department of Oral Diagnosis, Oral Pathology Division, Piracicaba Dental School, University of Campinas, SP-13414-903, Piracicaba, São Paulo, Brazil;Department of Oral and Maxillofacial Diseases, University of Helsinki, FI-00014, Helsinki, Finland;Faculty of Biochemistry and Molecular Medicine, University of Oulu, FI-90014, Oulu, Finland;Proteomics Core Facility, Biocenter Oulu, University of Oulu, FI-90014, Oulu, Finland;Laboratório Nacional de Biociências, LNBio, CNPEM, SP-13083-970, Campinas, Brazil;

关键词: Tumor microenvironment matrix;    Invasion;    Migration;    Hanging drop;    Colony formation;    Spheroid formation;    Capillary formation;   

DOI  :  10.1186/s12885-015-1944-z

 received in 2015-07-01, accepted in 2015-11-19,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe composition of the matrix molecules is important in in vitro cell culture experiments of e.g. human cancer invasion and vessel formation. Currently, the mouse Engelbreth-Holm-Swarm (EHS) sarcoma -derived products, such as Matrigel®, are the most commonly used tumor microenvironment (TME) mimicking matrices for experimental studies. However, since Matrigel® is non-human in origin, its molecular composition does not accurately simulate human TME. We have previously described a solid 3D organotypic myoma disc invasion assay, which is derived from human uterus benign leiomyoma tumor. Here, we describe the preparation and analyses of a processed, gelatinous leiomyoma matrix, named Myogel.MethodsA total protein extract, Myogel, was formulated from myoma. The protein contents of Myogel were characterized and its composition and properties compared with a commercial mouse Matrigel®. Myogel was tested and compared to Matrigel® in human cell adhesion, migration, invasion, colony formation, spheroid culture and vessel formation experiments, as well as in a 3D hanging drop video image analysis.ResultsWe demonstrated that only 34 % of Myogel’s molecular content was similar to Matrigel®. All test results showed that Myogel was comparable with Matrigel®, and when mixed with low-melting agarose (Myogel-LMA) it was superior to Matrigel® in in vitro Transwell® invasion and capillary formation assays.ConclusionsIn conclusion, we have developed a novel Myogel TME matrix, which is recommended for in vitro human cell culture experiments since it closely mimics the human tumor microenvironment of solid cancers.

【 授权许可】

CC BY   
© Salo et al. 2015