Potential serodiagnostic markers for Q fever identified in Coxiella burnetiiby immunoproteomic and protein microarray approaches

BMC Microbiology

Research Article

Stephen Graves¹  John Stenos¹  Xiaolu Xiong²  Xile Wang²  Bohai Wen² 

[1] Australian Rickettsial Reference Laboratory, Barwon Health, Geelong Hospital, 3220, Geelong, Victoria, Australia;State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Dong-dia-jie, 100071, Beijing, China;

关键词: Patient Seron;    Protein Microarray;    Average Fluorescence Intensity;    Coxiella Burnetii;    Fever Patient;   

DOI  :  10.1186/1471-2180-12-35

 received in 2011-09-30, accepted in 2012-03-16,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundCoxiella burnetii is the etiological agent of Q fever. The clinical diagnosis of Q fever is mainly based on several serological tests. These tests all need Coxiella organisms which are difficult and hazardous to culture and purify.ResultsAn immunoproteomic study of C. burnetii Xinqiao strain isolated in China was conducted with the sera from experimentally infected BALB/c mice and Q fever patients. Twenty of whole proteins of Xinqiao recognized by the infection sera were identified by mass spectrometry. Nineteen of the 20 proteins were successfully expressed in Escherichia coli and used to fabricate a microarray which was probed with Q fever patient sera. As a result, GroEL, YbgF, RplL, Mip, OmpH, Com1, and Dnak were recognized as major seroreactive antigens. The major seroreactive proteins were fabricated in a small microarray and further analyzed with the sera of patients with rickettsial spotted fever, Legionella pneumonia or streptococcal pneumonia. In this analysis, these proteins showed fewer cross-reactions with the tested sera.ConclusionsOur results demonstrate that these 7 Coxiella proteins gave a modest sensitivity and specificity for recognizing of Q fever patient sera, suggesting that they are potential serodiagnostic markers for Q fever.

【 授权许可】

Unknown   
© Xiong et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.