Nutrient control of eukaryote cell growth: a systems biology study in yeast

BMC Biology

Research Article

Alex Gutteridge¹  Pınar Pir¹  Stephen G Oliver¹  Juan I Castrillo¹  Philip D Charles¹  Kathryn S Lilley¹ 

[1] Cambridge Systems Biology Centre & Department of Biochemistry, University of Cambridge, Sanger Building, 80 Tennis Court Road, CB2 1GA, Cambridge, UK;

关键词: Gene Ontology;    Trehalose;    Nutrient Limitation;    Nitrogen Limitation;    Increase Growth Rate;   

DOI  :  10.1186/1741-7007-8-68

 received in 2010-04-08, accepted in 2010-05-24,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundTo elucidate the biological processes affected by changes in growth rate and nutrient availability, we have performed a comprehensive analysis of the transcriptome, proteome and metabolome responses of chemostat cultures of the yeast, Saccharomyces cerevisiae, growing at a range of growth rates and in four different nutrient-limiting conditions.ResultsWe find significant changes in expression for many genes in each of the four nutrient-limited conditions tested. We also observe several processes that respond differently to changes in growth rate and are specific to each nutrient-limiting condition. These include carbohydrate storage, mitochondrial function, ribosome synthesis, and phosphate transport. Integrating transcriptome data with proteome measurements allows us to identify previously unrecognized examples of post-transcriptional regulation in response to both nutrient and growth-rate signals.ConclusionsOur results emphasize the unique properties of carbon metabolism and the carbon substrate, the limitation of which induces significant changes in gene regulation at the transcriptional and post-transcriptional level, as well as altering how many genes respond to growth rate. By comparison, the responses to growth limitation by other nutrients involve a smaller set of genes that participate in specific pathways.See associated commentary http://www.biomedcentral.com/1741-7007/8/62

【 授权许可】

Unknown   
© Gutteridge et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.