Isoferulic acid prevents methylglyoxal-induced protein glycation and DNA damage by free radical scavenging activity

BMC Complementary and Alternative Medicine

Research Article

Catherine B. Chan¹  Tanyawan Suantawee²  Thavaree Thilavech²  Aramsri Meeprom³  Weerachat Sompong⁴  Sirichai Adisakwattana⁵ 

[1] Departments of Physiology and Agricultural, Food and Nutritional Sciences, University of Alberta, Edmonton, T6G 2R3, Alberta, Canada;Program in Biomedical Sciences, Graduate School, Chulalongkorn University, 10330, Bangkok, Thailand;Research Group of Herbal Medicine for Prevention and Therapeutic of Metabolic diseases, Department of Nutrition and Dietetics, Faculty of Allied Health Sciences, Chulalongkorn University, 10330, Bangkok, Thailand;Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, 10330, Bangkok, Thailand;Research Group of Herbal Medicine for Prevention and Therapeutic of Metabolic diseases, Department of Nutrition and Dietetics, Faculty of Allied Health Sciences, Chulalongkorn University, 10330, Bangkok, Thailand;Research Group of Herbal Medicine for Prevention and Therapeutic of Metabolic diseases, Department of Nutrition and Dietetics, Faculty of Allied Health Sciences, Chulalongkorn University, 10330, Bangkok, Thailand;Research Group of Herbal Medicine for Prevention and Therapeutic of Metabolic diseases, Department of Nutrition and Dietetics, Faculty of Allied Health Sciences, Chulalongkorn University, 10330, Bangkok, Thailand;Department of Nutrition and Dietetics, Faculty of Allied Health Sciences, Chulalongkorn University, 10330, Bangkok, Thailand;

关键词: Isoferulic acid;    Methylglyoxal;    Advanced glycation end products;    DNA damage;    Oxidation;   

DOI  :  10.1186/s12906-015-0874-2

 received in 2015-07-02, accepted in 2015-09-23,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundIsoferulic acid (IFA), a naturally occurring cinnamic acid derivative, is a main active ingredient of the rhizoma of Cimicifuga dahurica. It has been shown various pharmacological activities. The aim of the study was to investigate the effect of IFA against MG-induced protein glycation and oxidative DNA damage. Free radical scavenging activity and the MGO-trapping abilities of IFA were also investigated.MethodsThe fluorescent MG-derived AGEs and non-fluorescent Nε-(carboxymethyl) lysine (Nε-CML) was measured using a spectrofluorometer and an enzyme linked immunosorbant assay (ELISA). Protein carbonyl content was used to detect protein oxidation. Gel electrophoresis was used to determine DNA damage. Superoxide anion radicals and hydroxyl radicals were determined using cytochrome c reduction assay and thiobarbituric acid reactive 2-deoxy-D-ribose oxidation products, respectively. The MG-trapping capacity was performed by HPLC.ResultsIFA (1.25–5 mM) inhibited the formation of fluorescent MG-derived AGEs, and Nε-CML, and protein carbonyl in bovine serum albumin. In addition, IFA (0.1–1 mM) also prevented MG/lysine-mediated oxidative DNA damage in the presence and absence of copper ion. The protective ability of IFA was directly correlated to inhibition of hydroxyl and superoxide anion radical generation during the reaction of MG and lysine. Most notably, IFA had no the directly trapping ability to MG.ConclusionsThe present results highlighted that free radical scavenging activity, but not the MG-trapping ability, is the mechanism of IFA for preventing MG-induced protein glycation and DNA damage.

【 授权许可】

CC BY   
© Meeprom et al. 2015