ITRAQ-based quantitative proteomic analysis of Cynops orientalis limb regeneration

BMC Genomics

Research Article

Wenjun Wang¹  Hanxue Zheng¹  Lu Yin¹  Mei Sun¹  Yuan Yu¹  Xin Xie¹  Jihong Cui¹  Wenguang Liu¹  Fulin Chen²  Jie Tang³ 

[1] Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 Taibai North Road, 710069, Xi’an, Shaanxi Province, People’s Republic of China;Provincial Key Laboratory of Biotechnology of Shaanxi, Northwest University, 229 Taibai North Road, 710069, Xi’an, Shaanxi Province, People’s Republic of China;Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 Taibai North Road, 710069, Xi’an, Shaanxi Province, People’s Republic of China;Provincial Key Laboratory of Biotechnology of Shaanxi, Northwest University, 229 Taibai North Road, 710069, Xi’an, Shaanxi Province, People’s Republic of China;Key Laboratory of Resource Biology and Modern Biotechnology in Western China, Ministry of Education, Northwest University, 229 Taibai North Road, 710069, Xi’an, Shaanxi Province, People’s Republic of China;Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 Taibai North Road, 710069, Xi’an, Shaanxi Province, People’s Republic of China;Shaanxi Institute of Zoology, 88 Xingqing Road, 710032, Xi’an, Shaanxi Province, People’s Republic of China;

关键词: Cynops orientalis;    iTRAQ;    Proteome;    Limb regeneration;   

DOI  :  10.1186/s12864-017-4125-4

 received in 2017-02-16, accepted in 2017-09-06,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundSalamanders regenerate their limbs after amputation. However, the molecular mechanism of this unique regeneration remains unclear. In this study, isobaric tags for relative and absolute quantification (iTRAQ) coupled with liquid chromatography tandem mass spectrometry (LC-MS/MS) was employed to quantitatively identify differentially expressed proteins in regenerating limbs 3, 7, 14, 30 and 42 days post amputation (dpa).ResultsOf 2636 proteins detected in total, 253 proteins were differentially expressed during different regeneration stages. Among these proteins, Asporin, Cadherin-13, Keratin, Collagen alpha-1(XI) and Titin were down-regulated. CAPG, Coronin-1A, AnnexinA1, Cathepsin B were up-regulated compared with the control. The identified proteins were further analyzed to obtain information about their expression patterns and functions in limb regeneration. Functional analysis indicated that the differentially expressed proteins were associated with wound healing, immune response, cellular process, metabolism and binding.ConclusionsThis work indicated that significant proteome alternations occurred during salamander limb regeneration. The results may provide fundamental knowledge to understand the mechanism of limb regeneration.

【 授权许可】

CC BY   
© The Author(s). 2017