Substantial reprogramming of the Eutrema salsugineum (Thellungiella salsuginea) transcriptome in response to UV and silver nitrate challenge

BMC Plant Biology

Article

Stefanie Mucha¹  Teresa M Müller¹  Erich Glawischnig¹  Dirk Walther²  Dirk K Hincha² 

[1] Lehrstuhl für Genetik, Technische Universität München, D-85354, Freising, Germany;Max-Planck-Institut für Molekulare Pflanzenphysiologie, 14476, Potsdam, Germany;

关键词: Eutrema salsugineum;    Thellungiella salsuginea;    Transcriptomics;    Glucosinolate biosynthesis;    Phytoalexin;   

DOI  :  10.1186/s12870-015-0506-5

 received in 2015-02-27, accepted in 2015-04-24,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundCruciferous plants synthesize a large variety of tryptophan-derived phytoalexins in response to pathogen infection, UV irradiation, or high dosages of heavy metals. The major phytoalexins of Eutrema salsugineum (Thellungiella salsuginea), which has recently been established as an extremophile model plant, are probably derivatives of indole glucosinolates, in contrast to Arabidopsis, which synthesizes characteristic camalexin from the glucosinolate precursor indole-3-acetaldoxime.ResultsThe transcriptional response of E. salsugineum to UV irradiation and AgNO3 was monitored by RNAseq and microarray analysis. Most transcripts (respectively 70% and 78%) were significantly differentially regulated and a large overlap between the two treatments was observed (54% of total). While core genes of the biosynthesis of aliphatic glucosinolates were repressed, tryptophan and indole glucosinolate biosynthetic genes, as well as defence-related WRKY transcription factors, were consistently upregulated. The putative Eutrema WRKY33 ortholog was functionally tested and shown to complement camalexin deficiency in Atwrky33 mutant.ConclusionsIn E. salsugineum, UV irradiation or heavy metal application resulted in substantial transcriptional reprogramming. Consistently induced genes of indole glucosinolate biosynthesis and modification will serve as candidate genes for the biosynthesis of Eutrema-specific phytoalexins.

【 授权许可】

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© Mucha et al. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.