A high density genetic map and QTL for agronomic and yield traits in Foxtail millet [Setaria italica (L.) P. Beauv.]

BMC Genomics

Research Article

Ruiyu Ren¹  Tianyu Yang¹  Kongjun Dong¹  Lei Zhang¹  Jihong He¹  Tianpeng Liu¹  Zhengsheng Zhang²  Xiaoqin Wang²  Man Li²  Mengzhu Huang²  Xueying Liu²  Xiaomei Fang²  Rui Liu² 

[1] Crop Research Institute, Gansu Academy of Agricultural Sciences, 730070, Lanzhou, Gansu, People’s Republic of China;Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, 400716, Chongqing, People’s Republic of China;

关键词: Genetic map;    QTL;    Agronomic traits;    Yield traits;    Setaria italica;   

DOI  :  10.1186/s12864-016-2628-z

 received in 2015-11-27, accepted in 2016-04-16,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundFoxtail millet [Setaria italica (L.) P. Beauv.], a crop of historical importance in China, has been adopted as a model crop for studying C-4 photosynthesis, stress biology and biofuel traits. Construction of a high density genetic map and identification of stable quantitative trait loci (QTL) lay the foundation for marker-assisted selection for agronomic traits and yield improvement.ResultA total of 10598 SSR markers were developed according to the reference genome sequence of foxtail millet cultivar ‘Yugu1’. A total of 1013 SSR markers showing polymorphism between Yugu1 and Longgu7 were used to genotype 167 individuals from a Yugu1 × Longgu7 F2 population, and a high density genetic map was constructed. The genetic map contained 1035 loci and spanned 1318.8 cM with an average distance of 1.27 cM between adjacent markers. Based on agronomic and yield traits identified in 2 years, 29 QTL were identified for 11 traits with combined analysis and single environment analysis. These QTL explained from 7.0 to 14.3 % of phenotypic variation. Favorable QTL alleles for peduncle length originated from Longgu7 whereas favorable alleles for the other traits originated from Yugu1 except for qLMS6.1.ConclusionsNew SSR markers, a high density genetic map and QTL identified for agronomic and yield traits lay the ground work for functional gene mapping, map-based cloning and marker-assisted selection in foxtail millet.

【 授权许可】

CC BY   
© Fang et al. 2016