Combined transcriptome and metabolome analyses to understand the dynamic responses of rice plants to attack by the rice stem borer Chilo suppressalis (Lepidoptera: Crambidae)

BMC Plant Biology

Research Article

Qingsong Liu¹  Xingyun Wang¹  Yunhe Li¹  Yufa Peng¹  Jörg Romeis²  Vered Tzin³ 

[1] State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China;Agroscope, Biosafety Research Group, Zurich, Switzerland;The French Associates Institute for Agriculture and Biotechnology of Drylands, The Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Sede Boqer, Israel;

关键词: Oryza sativa;    Induced response;    Next generation sequencing;    Plant-insect interactions;    Phytohormones;    Phenylpropanoids;    Carbohydrates;    Amino acids;    Terpenoids;   

DOI  :  10.1186/s12870-016-0946-6

 received in 2016-07-26, accepted in 2016-11-23,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundRice (Oryza sativa L.), which is a staple food for more than half of the world’s population, is frequently attacked by herbivorous insects, including the rice stem borer, Chilo suppressalis. C. suppressalis substantially reduces rice yields in temperate regions of Asia, but little is known about how rice plants defend themselves against this herbivore at molecular and biochemical level.ResultsIn the current study, we combined next-generation RNA sequencing and metabolomics techniques to investigate the changes in gene expression and in metabolic processes in rice plants that had been continuously fed by C. suppressalis larvae for different durations (0, 24, 48, 72, and 96 h). Furthermore, the data were validated using quantitative real-time PCR. There were 4,729 genes and 151 metabolites differently regulated when rice plants were damaged by C. suppressalis larvae. Further analyses showed that defense-related phytohormones, transcript factors, shikimate-mediated and terpenoid-related secondary metabolism were activated, whereas the growth-related counterparts were suppressed by C. suppressalis feeding. The activated defense was fueled by catabolism of energy storage compounds such as monosaccharides, which meanwhile resulted in the increased levels of metabolites that were involved in rice plant defense response. Comparable analyses showed a correspondence between transcript patterns and metabolite profiles.ConclusionThe current findings greatly enhance our understanding of the mechanisms of induced defense response in rice plants against C. suppressalis infestation at molecular and biochemical levels, and will provide clues for development of insect-resistant rice varieties.

【 授权许可】

CC BY   
© The Author(s). 2016