Differential transcriptome analysis of glandular and filamentous trichomes in Artemisia annua

BMC Plant Biology

Research Article

Alain Goossens¹  Mado L Vandewoestyne²  Christophe MF Van Neste²  Dieter LD Deforce²  Filip CW Van Nieuwerburgh²  Sandra SA Soetaert²  Steven R Head³ 

[1] Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, 9052, Ghent, Belgium;Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium;Next Generation Sequencing Core, The Scripps Research Institute, 10550N. Torrey Pines Rd, 92037, La Jolla, CA, United States of America;

关键词: Artemisia annua;    Artemisinin;    RNASeq;    Glandular trichomes;    Filamentous trichomes;    Laser microdissection pressure catapulting;    MEP pathway;    Mevalonate pathway;    Lipid biosynthesis;    Terpene biosynthesis;   

DOI  :  10.1186/1471-2229-13-220

 received in 2013-03-08, accepted in 2013-12-12,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe medicinal plant Artemisia annua is covered with filamentous trichomes and glandular, artemisinin producing trichomes. A high artemisinin supply is needed at a reduced cost for treating malaria. Artemisinin production in bioreactors can be facilitated if a better insight is obtained in the biosynthesis of artemisinin and other metabolites. Therefore, metabolic activities of glandular and filamentous trichomes were investigated at the transcriptome level.ResultsBy laser pressure catapulting, glandular and filamentous trichomes as well as apical and sub-apical cells from glandular trichomes were collected and their transcriptome was sequenced using Illumina RNA-Seq. A de novo transcriptome was assembled (Trinity) and studied with a differential expression analysis (edgeR).A comparison of the transcriptome from glandular and filamentous trichomes shows that MEP, MVA, most terpene and lipid biosynthesis pathways are significantly upregulated in glandular trichomes. Conversely, some transcripts coding for specific sesquiterpenoid and triterpenoid enzymes such as 8-epi-cedrol synthase and an uncharacterized oxidosqualene cyclase were significantly upregulated in filamentous trichomes. All known artemisinin biosynthesis genes are upregulated in glandular trichomes and were detected in both the apical and sub-apical cells of the glandular trichomes. No significant differential expression could be observed between the apical and sub-apical cells.ConclusionsOur results underscore the vast metabolic capacities of A. annua glandular trichomes but nonetheless point to the existence of specific terpene metabolic pathways in the filamentous trichomes. Candidate genes that might be involved in artemisinin biosynthesis are proposed based on their putative function and their differential expression level.

【 授权许可】

Unknown   
© Soetaert et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.