Indication for Co-evolution of Lactobacillus johnsonii with its hosts

BMC Microbiology

Research Article

Keren Buhnik-Rosenblau¹  Vera Matsko-Efimov¹  Yael Danin-Poleg¹  Yechezkel Kashi¹  Minju Jung²  Heuynkil Shin² 

[1] Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, Israel;School of Life Sciences, Handong Global University, 791-708, Pohang, Gyungbuk, Korea;

关键词: Lactobacillus;    Lactic Acid Bacterium;    Terminal Restriction Fragment Length Polymorphism;    Simple Sequence Repeat Locus;    Unweighted Pair Group Method With Arithmetic;   

DOI  :  10.1186/1471-2180-12-149

 received in 2011-12-29, accepted in 2012-06-29,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThe intestinal microbiota, composed of complex bacterial populations, is host-specific and affected by environmental factors as well as host genetics. One important bacterial group is the lactic acid bacteria (LAB), which include many health-promoting strains. Here, we studied the genetic variation within a potentially probiotic LAB species, Lactobacillus johnsonii, isolated from various hosts.ResultsA wide survey of 104 fecal samples was carried out for the isolation of L. johnsonii. As part of the isolation procedure, terminal restriction fragment length polymorphism (tRFLP) was performed to identify L. johnsonii within a selected narrow spectrum of fecal LAB. The tRFLP results showed host specificity of two bacterial species, the Enterococcus faecium species cluster and Lactobacillus intestinalis, to different host taxonomic groups while the appearance of L. johnsonii and E. faecalis was not correlated with any taxonomic group. The survey ultimately resulted in the isolation of L. johnsonii from few host species. The genetic variation among the 47 L. johnsonii strains isolated from the various hosts was analyzed based on variation at simple sequence repeats (SSR) loci and multi-locus sequence typing (MLST) of conserved hypothetical genes. The genetic relationships among the strains inferred by each of the methods were similar, revealing three different clusters of L. johnsonii strains, each cluster consisting of strains from a different host, i.e. chickens, humans or mice.ConclusionsOur typing results support phylogenetic separation of L. johnsonii strains isolated from different animal hosts, suggesting specificity of L. johnsonii strains to their hosts. Taken together with the tRFLP results, that indicated the association of specific LAB species with the host taxonomy, our study supports co-evolution of the host and its intestinal lactic acid bacteria.

【 授权许可】

Unknown   
© Buhnik-Rosenblau et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.