microRNA analysis of gastric cancer patients from Saudi Arabian population

BMC Genomics

Research

Muhammad I. Naseer¹  Mohammed H. Al-Qahtani¹  Adel M. Abuzenadah²  Ali Sawan³  Asif A. Jiman-Fatani⁴  Muhammad Yasir⁵  Fehmida Bibi⁵  Sana Akhtar Alvi⁵  Esam I. Azhar⁶ 

[1] Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, 21589, Jeddah, Saudi Arabia;Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, 21589, Jeddah, Saudi Arabia;KACST Technology Innovation Center in Personalized Medicine, King Abdulaziz University, P.O. Box 80216, 21589, Jeddah, Kingdom of Saudi Arabia;Department of Anatomical Pathology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia;Department of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia;Special Infectious Agents Unit, King Fahd Medical Research Centre, King Abdulaziz University, 21589, Jeddah, Kingdom of Saudi Arabia;Special Infectious Agents Unit, King Fahd Medical Research Centre, King Abdulaziz University, 21589, Jeddah, Kingdom of Saudi Arabia;Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia;

关键词: Gastric cancer;    MicroRNAs;    Microarray;    Real time PCR;    Diagnostic biomarker;   

DOI  :  10.1186/s12864-016-3090-7

来源: Springer

【 摘 要 】

BackgroundThe role of small non-coding microRNAs (miRNAs) in several types of cancer has been evident. However, its expression studies have never been performed in gastric cancer (GC) patients from Saudi population. First time this study was conducted to identify miRNAs that are differentially expressed in GC patients compared with normal controls.MethodsWe investigated the role of miRNAs in GC patients using formalin-fixed paraffin-embedded (FFPE) tissues of 34 samples from GC patients (early stage = 7 and late-stage = 26) and 15 from normal control. We have used miRNA microarray analysis and validated the results by Real-time quantitative PCR (RT-qPCR).ResultsWe obtained data of 1082 expressed genes, from cancer tissues and noncancerous tissues (49 samples in total). Where 129 genes were up-regulated (P > 0.05) and 953 genes (P > 0.05) were down-regulated in 49 FFPE tissue samples. Only 33 miRNAs had significant expression in early and late-stage cancer tissues. After candidate miRNAs were selected, RT-qPCR further confirmed that four miRNAs (hsa-miR-200c-3p, hsa-miR-3613, hsa-miR-27b-3p, hsa-miR-4668-5p) were significantly aberrant in GC tissues compared to the normal gastric tissues.ConclusionsIn this study we provide miRNAs profile of GC where many miRNAs showed aberrant expression from normal tissues, suggesting their involvement in the development and progression of gastric cancer. In early and late-stage miR-200c-3p showed significant down regulation as compare to control samples. Many of miRNAs reported in our study showing up-regulation are new and not reported before may be due to population difference. In conclusion, our results suggest that miR-200c-3p had potential to use as diagnostic biomarker for distinguishing GC patients from normal individuals and can be used for diagnosis of cancer at early stage.

【 授权许可】

CC BY   
© The Author(s). 2016