The phylogenomic analysis of the anaphase promoting complex and its targets points to complex and modern-like control of the cell cycle in the last common ancestor of eukaryotes

BMC Evolutionary Biology

Research Article

Aurélie Trilles¹  Laura Eme¹  Céline Brochier-Armanet²  David Moreira³ 

[1] Aix-Marseille Université, Laboratoire de Chimie Bactérienne, UPR CNRS 9043, Marseille, France;Aix-Marseille Université, Laboratoire de Chimie Bactérienne, UPR CNRS 9043, Marseille, France;Université de Lyon; Université Lyon 1; CNRS; UMR5558, Laboratoire de Biométrie et Biologie Evolutive, 43 boulevard du 11 novembre 1918, Villeurbanne, France;Unité d'Ecologie, Systématique et Evolution, Université Paris-Sud, UMR CNRS 8079, Orsay, France;

关键词: Anaphase Promoting Complex;    Cohesin Complex;    Phylogeny;    Eukaryotes;    LECA;    Evolution;   

DOI  :  10.1186/1471-2148-11-265

 received in 2011-04-18, accepted in 2011-09-23,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe Anaphase Promoting Complex or Cyclosome (APC/C) is the largest member of the ubiquitin ligase [E3] family. It plays a crucial role in the control of the cell cycle and cell proliferation by mediating the proteolysis of key components by the proteasome. APC/C is made of a dozen subunits that assemble into a large complex of ~1.5 MDa, which interacts with various cofactors and targets.ResultsUsing comparative genomic and phylogenetic approaches, we showed that 24 out of 37 known APC/C subunits, adaptors/co-activators and main targets, were already present in the Last Eukaryotic Common Ancestor (LECA) and were well conserved to a few exceptions in all present-day eukaryotic lineages. The phylogenetic analysis of the 24 components inferred to be present in LECA showed that they contain a reliable phylogenetic signal to reconstruct the phylogeny of the domain Eucarya.ConclusionsTaken together our analyses indicated that LECA had a complex and highly controlled modern-like cell cycle. Moreover, we showed that, despite what is generally assumed, proteins involved in housekeeping cellular functions may be a good complement to informational genes to study the phylogeny of eukaryotes.

【 授权许可】

Unknown   
© Eme et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.