The phylogenomic analysis of the anaphase promoting complex and its targets points to complex and modern-like control of the cell cycle in the last common ancestor of eukaryotes

BMC Evolutionary Biology

Laura Eme¹  Aurlie Trilles² 

[1] Aix-Marseille Universit, Laboratoire de Chimie Bactrienne, Marseille, France;Unit dEcologie, Systmatique et Evolution, Orsay, France

关键词: Anaphase Promoting Complex;    Cohesin Complex;    Phylogeny;    Eukaryotes;    LECA;    Evolution;   

DOI  :  10.1186/1471-2148-11-265

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

The Anaphase Promoting Complex or Cyclosome (APC/C) is the largest member of the ubiquitin ligase [E3] family. It plays a crucial role in the control of the cell cycle and cell proliferation by mediating the proteolysis of key components by the proteasome. APC/C is made of a dozen subunits that assemble into a large complex of ~1.5 MDa, which interacts with various cofactors and targets. Using comparative genomic and phylogenetic approaches, we showed that 24 out of 37 known APC/C subunits, adaptors/co-activators and main targets, were already present in the Last Eukaryotic Common Ancestor (LECA) and were well conserved to a few exceptions in all present-day eukaryotic lineages. The phylogenetic analysis of the 24 components inferred to be present in LECA showed that they contain a reliable phylogenetic signal to reconstruct the phylogeny of the domain Eucarya. Taken together our analyses indicated that LECA had a complex and highly controlled modern-like cell cycle. Moreover, we showed that, despite what is generally assumed, proteins involved in housekeeping cellular functions may be a good complement to informational genes to study the phylogeny of eukaryotes.

【 授权许可】

CC BY