Carbohydrate utilization and metabolism is highly differentiated in Agaricus bisporus

BMC Genomics

Research Article

Miaomiao Zhou¹  Ad Wiebenga¹  Aleksandrina Patyshakuliyeva¹  Joost van den Brink¹  Ronald P de Vries²  Birgit S Gruben²  Evy Battaglia²  Daniel C Eastwood³  Miia R Mäkelä⁴  Kerry S Burton⁵  Francis Martin⁶  Annegret Kohler⁶  Marina Nadal⁷  Pedro M Coutinho⁸  Bernard Henrissat⁸  Adam Baker⁹  Wouter de Bruijn^1,10  Edita Jurak^1,10  Mirjam Kabel^1,10  Harry Gruppen^1,10  Michael P Challen^1,11 

[1] CBS-KNAW Fungal Biodiversity Centre, 3584 CT, Uppsalalaan 8, Utrecht, The Netherlands;CBS-KNAW Fungal Biodiversity Centre, 3584 CT, Uppsalalaan 8, Utrecht, The Netherlands;Microbiology, Utrecht University, 3584 CH, Padualaan 8, Utrecht, The Netherlands;College of Science, University of Swansea, SA2 8PP, Singleton Park, Swansea, UK;Department of Food and Environmental Sciences, University of Helsinki, P. O. Box 56, 00014, Helsinki, Finland;East Malling Research, New Road, East Malling, ME19 6BJ, Kent, UK;INRA, UMR1136 INRA/UHP, Interactions Arbres/ Micro-organismes, 54280, Centre de Nancy, Champenoux, France;Microbiology, Utrecht University, 3584 CH, Padualaan 8, Utrecht, The Netherlands;UMR 6098 CNRS–Universités Aix-Marseille I and II, 13288, Marseille Cedex 9, France;University of Warwick, CV35 9EF, Warwick, Wellesbourne, UK;Wageningen University, Laboratory of Food Chemistry, 6703 HD, Bomenweg 2, Wageningen, The Netherlands;Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, OX3 7BN, Oxford, UK;

关键词: Trehalose;    Wheat Straw;    Fruiting Body;    Pentose Phosphate Pathway;    Fungal Cell Wall;   

DOI  :  10.1186/1471-2164-14-663

 received in 2013-04-29, accepted in 2013-09-26,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundAgaricus bisporus is commercially grown on compost, in which the available carbon sources consist mainly of plant-derived polysaccharides that are built out of various different constituent monosaccharides. The major constituent monosaccharides of these polysaccharides are glucose, xylose, and arabinose, while smaller amounts of galactose, glucuronic acid, rhamnose and mannose are also present.ResultsIn this study, genes encoding putative enzymes from carbon metabolism were identified and their expression was studied in different growth stages of A. bisporus. We correlated the expression of genes encoding plant and fungal polysaccharide modifying enzymes identified in the A. bisporus genome to the soluble carbohydrates and the composition of mycelium grown compost, casing layer and fruiting bodies.ConclusionsThe compost grown vegetative mycelium of A. bisporus consumes a wide variety of monosaccharides. However, in fruiting bodies only hexose catabolism occurs, and no accumulation of other sugars was observed. This suggests that only hexoses or their conversion products are transported from the vegetative mycelium to the fruiting body, while the other sugars likely provide energy for growth and maintenance of the vegetative mycelium. Clear correlations were found between expression of the genes and composition of carbohydrates. Genes encoding plant cell wall polysaccharide degrading enzymes were mainly expressed in compost-grown mycelium, and largely absent in fruiting bodies. In contrast, genes encoding fungal cell wall polysaccharide modifying enzymes were expressed in both fruiting bodies and vegetative mycelium, but different gene sets were expressed in these samples.

【 授权许可】

Unknown   
© Patyshakuliyeva et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.