RAPD-PCR reveals genetic polymorphism among Leishmania major strains from Tunisian patients

BMC Infectious Diseases

Research Article

Amor Zaatour¹  Jihene Bettaieb¹  Rihab Yazidi¹  Wissem Ghawar¹  Sana Châabane¹  Kaouther Jaouadi¹  Afif Ben Salah¹ 

[1] Department of Medical Epidemiology, Laboratory of Transmission, Control and Immunobiology of Infections (LR11IPT02), Pasteur Institute of Tunis, 13 Place Pasteur BP-74, 1002, Tunis, Belvedere, Tunisia;

关键词: Leishmania major;    Genetic Polymorphism;    RAPDPCR;    Tunisia;   

DOI  :  10.1186/s12879-015-1010-0

 received in 2014-12-04, accepted in 2015-07-06,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundZoonotic cutaneous leishmaniasis caused by Leishmania (L.) major is endemoepidemic in the Center and South of Tunisia. The clinical course of the disease varies widely among different patients and geographic regions. Although genetic diversity in L. major parasites has been suggested as a potential factor influencing their pathogenic variability, little information on genetic polymorphism among L. major strains is available in the literature. This work aimed to estimate the genetic variability within different isolates of L. major.MethodsOur sample comprised 39 isolates (confirmed as L. major by restriction fragment length polymorphism typing) from patients experiencing the same clinical manifestations but living in different regions of Tunisia where L. major is endemic. Random amplified polymorphic DNA (RAPD) PCR marker polymorphism was estimated by calculating Nei and Li’s genetic distances and by an analysis of molecular variance (AMOVA).ResultsAnalysis of the genetic diversity among the isolates revealed a high level of polymorphism (43 %) among them. AMOVA indicated that the highest variability (99 %) existed within the study regions.ConclusionsOur results revealed a heterogeneous genetic profile for L. major with similar clinical manifestations occurring within the different geographical regions. Additional L. major isolates from patients, insect vectors, and reservoir hosts from different endemic foci should be collected for further analysis.

【 授权许可】

Unknown   
© Yazidi et al. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.