Global analysis of the differentially expressed miRNAs of prostate cancer in Chinese patients

BMC Genomics

Research Article

Xingyin Liu¹  Jia-hong Chen²  Fu-neng Jiang²  Zhao-dong Han²  Xin Fu²  Qi-shan Dai²  Ye-han Deng²  Hui-chan He²  Zhuo-yuan Lin²  Wei-de Zhong³  Chao Cai⁴  Xiao-hui Ling⁴  Guo-qiang Qin⁴ 

[1] Department of Genetics, Albert Einstein College of Medicine, 10461, Bronx, New York, USA;Department of Urology, Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First People’s Hospital, Guangzhou Medical University, 510180, Guangzhou, China;Department of Urology, Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First People’s Hospital, Guangzhou Medical University, 510180, Guangzhou, China;Guangdong Provincial Institute of Nephrology, Southern Medical University, 510515, Guangzhou, China;Urology Key Laboratory of Guangdong Province, Guangzhou Medical University, 510230, Guangzhou, China;Guangdong Provincial Institute of Nephrology, Southern Medical University, 510515, Guangzhou, China;

关键词: Prostate cancer;    miRNA expression profile;    miR-374b;    mRNA expression profile;   

DOI  :  10.1186/1471-2164-14-757

 received in 2013-02-27, accepted in 2013-10-24,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundOur recent study showed the global physiological function of the differentially expressed genes of prostate cancer in Chinese patients was different from that of other non-Chinese populations. microRNA are estimated to regulate the expression of greater than 60% of all protein-coding genes. To further investigate the global association between the transcript abundance of miRNAs and their target mRNAs in Chinese patients, we used microRNA microarray approach combined with bioinformatics and clinical-pathological assay to investigate the miRNA profile and evaluate the potential of miRNAs as diagnostic and prognostic markers in Chinese patients.ResultsA total of 28 miRNAs (fold change ≥1.5; P ≤ 0.05) were differentially expressed between tumor tissue and adjacent benign tissue of 4 prostate cancer patients.10 top Differentially expressed miRNAs were validated by qRT-PCR using all 20 tissue pairs. Compared to the miRNA profile of non-Chinese populations, the current study showed that miR-23b, miR-220, miR-221, miR-222, and miR-205 maybe common critical therapeutic targets in different populations. The integrated analysis for mRNA microarray and miRNA microarray showed the effects of specifically inhibiting and/or enhancing the function of miRNAs on the gene transcription level. The current studies also identified 15 specific expressed miRNAs in Chinese patients. The clinical feature statistics revealed that miR-374b and miR-19a have significant correlations with clinical-pathological features in Chinese patients.ConclusionsOur findings showed Chinese prostate cancer patients have a common and specific miRNA expression profile compared with non-Chinese populations. The miR-374b is down-regulated in prostate cancer tissue, and it can be identified as an independent predictor of biochemical recurrence-free survival.

【 授权许可】

Unknown   
© He et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.