Reactive oxygen species and transcript analysis upon excess light treatment in wild-type Arabidopsis thaliana vs a photosensitive mutant lacking zeaxanthin and lutein

BMC Plant Biology

Research Article

Alessio Aprile¹  Luigi Cattivelli¹  Alessandro Alboresi²  Luca Dall'Osto²  Roberto Bassi²  Enrica Roncaglia³  Petronia Carillo⁴ 

[1] CRA Centro di Ricerca per la Genomica, Via San Protaso 302, 29017, Fiorenzuola d'Arda, Italy;Dipartimento di Biotecnologie, Università di Verona, Strada Le Grazie 15, I - 37134, Verona, Italy;Dipartimento di Scienze Biomediche, Università di Modena e Reggio Emilia, Via Campi 287, 41100, Modena, Italy;Dipartimento di Scienze della Vita, Seconda Università degli Studi di Napoli, Via Vivaldi 43, Caserta, Italy;

关键词: Xanthophyll;    Zeaxanthin;    Excess Light;    Reactive Oxygen Species Species;    Singlet Oxygen Sensor Green;   

DOI  :  10.1186/1471-2229-11-62

 received in 2011-01-28, accepted in 2011-04-11,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundReactive oxygen species (ROS) are unavoidable by-products of oxygenic photosynthesis, causing progressive oxidative damage and ultimately cell death. Despite their destructive activity they are also signalling molecules, priming the acclimatory response to stress stimuli.ResultsTo investigate this role further, we exposed wild type Arabidopsis thaliana plants and the double mutant npq1lut2 to excess light. The mutant does not produce the xanthophylls lutein and zeaxanthin, whose key roles include ROS scavenging and prevention of ROS synthesis. Biochemical analysis revealed that singlet oxygen (1O2) accumulated to higher levels in the mutant while other ROS were unaffected, allowing to define the transcriptomic signature of the acclimatory response mediated by 1O2 which is enhanced by the lack of these xanthophylls species. The group of genes differentially regulated in npq1lut2 is enriched in sequences encoding chloroplast proteins involved in cell protection against the damaging effect of ROS. Among the early fine-tuned components, are proteins involved in tetrapyrrole biosynthesis, chlorophyll catabolism, protein import, folding and turnover, synthesis and membrane insertion of photosynthetic subunits. Up to now, the flu mutant was the only biological system adopted to define the regulation of gene expression by 1O2. In this work, we propose the use of mutants accumulating 1O2 by mechanisms different from those activated in flu to better identify ROS signalling.ConclusionsWe propose that the lack of zeaxanthin and lutein leads to 1O2 accumulation and this represents a signalling pathway in the early stages of stress acclimation, beside the response to ADP/ATP ratio and to the redox state of both plastoquinone pool. Chloroplasts respond to 1O2 accumulation by undergoing a significant change in composition and function towards a fast acclimatory response. The physiological implications of this signalling specificity are discussed.

【 授权许可】

Unknown   
© Alboresi et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.