The neoxanthin binding site of the major light harvesting complex (LHCII) from higher plants

FEBS Letters

Varotto, Claudio¹  Croce, Roberta¹  Remelli, Rosaria¹  Bassi, Roberto¹  Breton, Jacques² 

[1] Università di Verona, Facoltà di Scienze MM.FF.NN., Biotecnologie Vegetali, Strada Le Grazie, 37134 Verona, Italy;Département de Biologie Cellulaire et Moléculaire, CEA-Saclay, 91191 Gif sur Yvette Cedex, France

关键词: Photosynthesis;    Xanthophyll;    Linear dichroism;    DM;    β-dodecyl maltoside;    Chl;    chlorophyll;    CP;    chlorophyll-protein;    c.m.c.;    critical micellar concentration;    EDTA;    ethylenediaminetetraacetic acid;    HEPES;    N-2-(hydroxy-ethyl)piperazine-N′-2-ethane-sulfonic acid;    LHCII;    light harvesting complex II;    LD;    linear dichroism;    Lute (L);    lutein;    Neo (N);    neoxanthin;    PAGE;    polyacrylamide gel electrophoresis;    PSII;    photosystem II;    SDS;    sodium dodecyl sulphate;    Viola (V);    violaxanthin;   

DOI  :  10.1016/S0014-5793(99)00907-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The localisation of the xanthophyll neoxanthin within the structure of the major light harvesting complex (LHCII) of higher plants has been investigated by site-directed mutagenesis and spectroscopic methods. Mutation analysis performed on pigment binding sites in different helix domains leads to selective loss of neoxanthin for mutations on helix C thus localising this pigment between the helix C and helix A/B domains. Recombinant proteins binding two lutein molecules per polypeptide but lacking neoxanthin have been used in order to determine the contribution of neoxanthin to the absorption and linear dichroism spectra. The data were used to derive the orientation of the neoxanthin transition moment, lying in the polyene chain, which was thus determined to form an angle of 57±1.5° with respect to the normal to the membrane plane where the protein is inserted. On the basis of these results we propose a model for the localisation of the carotenoid site in the LHCII structure which is still unresolved.

【 授权许可】

Unknown