Preliminary antimycobacterial study on selected Turkish plants (Lamiaceae) against Mycobacterium tuberculosis and search for some phenolic constituents

BMC Complementary and Alternative Medicine

Research Article

Tülin Askun¹  Fatih Satil¹  Seyma Modanlioglu¹  Hatice Aydeniz¹  Emmanuel Mouafo Tekwu² 

[1] Department of Biology, Faculty of Sciences and Arts, University of Balikesir, 10145, Cagis Campus, Balikesir, Turkey;Department of Biology, Faculty of Sciences and Arts, University of Balikesir, 10145, Cagis Campus, Balikesir, Turkey;Laboratory for Tuberculosis Research (LTR), Biotechnology Centre, University of Yaoundé I, P.O. Box 8936, Yaoundé, Cameroon;

关键词: Mycobacterium tuberculosis;    Antimycobacterial activity;    Satureja aintabensis;    Thymus sipthorpii,;    Phenolic;    Flavonoid;   

DOI  :  10.1186/1472-6882-13-365

 received in 2013-09-14, accepted in 2013-12-13,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe global resurgence of tuberculosis is a significant threat. Lamiaceae members have been used in folk remedies for centuries. This study was designed to assess the in-vitro antimycobacterial activity of eighteen crude extracts from six plants (Lamiaceae) and to characterize their phenolic and flavonoid compounds.MethodsSix Turkish medicinal plants of the family Lamiaceae (Stachys tmolea Boiss., Stachys thirkei C. Koch, Ballota acetabulosa (L.) Benth., Thymus sipthorpii Benth., Satureja aintabensis P.H. Davis, and Micromeria juliana (L.) Benth. ex Reich.) were collected in 2009 – 2010. Dried and crushed plant samples were subjected to sequential extraction with petroleum ether, ethyl acetate, and methanol in order of increasing polarity. A broth microdilution method was employed to screen extracts against four mycobacterial strains of Mycobacterium tuberculosis. Phenolic and flavonoid compounds were characterized by liquid chromatography–mass spectrometry.ResultsS. aintabensis, T. sibthorpii, and M. juliana were found to develop considerable activity against the four strains of M. tuberculosis with the minimal inhibitory concentrations value of 12.5-100 μg/ml. S. aintabensis and T. sibthorpii extracts killed M. tuberculosis with the minimum bactericidal concentration value of 50–800 μg/ml. On the basis of these prominent antimycobacterial activity, we suggest that they could be a source of natural anti-tuberculosis agents.ConclusionS. aintabensis and T. sibthorpii showed activity by killing Mycobacteria strains. The major phenolic compound was rosmarinic for T. sibthorpii and S. aintabensis. Flavonoids might be “a modal” for the drug design.

【 授权许可】

Unknown   
© Askun et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.