Epithelial response to a high-protein diet in rat colon

BMC Genomics

Research Article

Marco Moroldo¹  Mireille Andriamihaja²  Lucie Armand²  Martin Beaumont²  Daniel Tomé²  François Blachier²  Annaïg Lan²  Marta Grauso²  Anne-Marie Davila²  Florence Jaffrézic³  Denis Laloë³ 

[1] CRB GADIE, INRA, 78350, Jouy-en-Josas, France;UMR Physiologie de la Nutrition et du Comportement Alimentaire, AgroParisTech, INRA, Université Paris-Saclay, 16 rue Claude Bernard, 75005, Paris, France;UMR1313 Génétique Animale et Biologie Intégrative, INRA, 78350, Jouy-en-Josas, France;

关键词: Epithelial cells;    Colon;    Dietary protein;    High-protein diet;    Transcriptome;    DNA damages;    Barrier function;    Mucus;    Epithelial renewal;   

DOI  :  10.1186/s12864-017-3514-z

 received in 2016-09-23, accepted in 2017-01-25,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundHigh-protein diets (HPD) alter the large intestine microbiota composition in association with a metabolic shift towards protein degradation. Some amino acid-derived metabolites produced by the colon bacteria are beneficial for the mucosa while others are deleterious at high concentrations. The aim of the present work was to define the colonic epithelial response to an HPD. Transcriptome profiling was performed on colonocytes of rats fed an HPD or an isocaloric normal-protein diet (NPD) for 2 weeks.ResultsThe HPD downregulated the expression of genes notably implicated in pathways related to cellular metabolism, NF-κB signaling, DNA repair, glutathione metabolism and cellular adhesion in colonocytes. In contrast, the HPD upregulated the expression of genes related to cell proliferation and chemical barrier function. These changes at the mRNA level in colonocytes were not associated with detrimental effects of the HPD on DNA integrity (comet assay), epithelium renewal (quantification of proliferation and apoptosis markers by immunohistochemistry and western blot) and colonic barrier integrity (Ussing chamber experiments).ConclusionThe modifications of the luminal environment after an HPD were associated with maintenance of the colonic homeostasis that might be the result of adaptive processes in the epithelium related to the observed transcriptional regulations.

【 授权许可】

CC BY   
© The Author(s). 2017