In vivo and in vitro evaluation of the effects of Urtica dioica and swimming activity on diabetic factors and pancreatic beta cells

BMC Complementary and Alternative Medicine

Research Article

Samad Akbarzadeh¹  Parviz Farzadinia²  Firouzeh Dehghan³  Ashril Yusof³  Mohamed Yousif Ibrahim⁴  Abbas Ranjbari⁵  Abdul Halim Mokhtar⁶  Bahman Tarverdizadeh⁷  Mohammad Ali Azarbayjani⁸  Reza Hajiaghaee⁹ 

[1] Biochemistry Department, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran;Biology and Anatomical Sciences, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran;Department of Exercise Science, Sports Centre, University Malaya, 50603, Kuala Lumpur, Malaysia;Department of Pharmacy, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia;Department of Physical Education, Sanandaj Farhangyan University, Sanandaj, Iran;Department of Sport Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia;Exercise Physiology Department, Faculty of Physical Education, Islamic Azad University, Bushehr Branch, Bushehr, Iran;Exercise Physiology Department, Faculty of Physical Education, Islamic Azad University, Central Tehran Branch, Tehran, Iran;Pharmacognosy and Pharmaceutic Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran;

关键词: Diabetes;    Urtica dioica;    Insulin resistance;    Cholesterol;    TG;    Pancreatic islet beta cells;    Swimming exercise;   

DOI  :  10.1186/s12906-016-1064-6

 received in 2015-07-29, accepted in 2016-02-23,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundUrtica dioica (UD) has been identified as a traditional herbal medicine. This study aimed to investigate the effect of UD extract and swimming activity on diabetic parameters through in vivo and in vitro experiments.MethodsAdult WKY male rats were randomly distributed in nine groups: intact control, diabetic control, diabetic + 625 mg/kg, 1.25 g/kg UD, diabetic + 100 mg/kg Metformin, diabetic + swimming, diabetic + swimming 625 mg/kg, 1.25 g/kg UD, and diabetic +100 mg/kg Metformin + swimming. The hearts of the animals were punctured, and blood samples were collected for biochemical analysis. The entire pancreas was exposed for histologic examination. The effect of UD on insulin secretion by RIN-5F cells in 6.25 or 12.5 mM glucose dose was examined. Glucose uptake by cultured L6 myotubes was determined.ResultsThe serum glucose concentration decreased, the insulin resistance and insulin sensitivity significantly increased in treated groups. These changes were more pronounced in the group that received UD extract and swimming training. Regeneration and less beta cell damage of Langerhans islets were observed in the treated groups. UD treatment increased insulin secretion in the RIN-5F cells and glucose uptake in the L6 myotubes cells.ConclusionsSwimming exercises accompanied by consuming UD aqueous extracts effectively improved diabetic parameters, repaired pancreatic tissues in streptozotocin-induced diabetics in vivo, and increased glucose uptake or insulin in UD-treated cells in vitro.

【 授权许可】

CC BY   
© Ranjbari et al. 2016