期刊论文详细信息
BMC Complementary and Alternative Medicine
Antimicrobial and antiproliferative activities of stingless bee Melipona scutellaris geopropolis
Research Article
Hyun Koo1  Masarahu Ikegaki2  Severino Matias de Alencar3  Pedro Luiz Rosalen4  Marcelo Franchin4  LíviaCâmaradeCarvalho Galvão4  Marcos Guilherme da Cunha4  João Ernesto de Carvalho5  AnaLúciaTascaGóis de Ruiz5 
[1] Center for Oral Biology, University of Rochester, 601 Elmwood Avenue, 14642, Rochester, NY, USA;College of Pharmaceutical Sciences, Federal University of Alfenas, 37130–000, Alfenas, MG, Brazil;Department of Agri-Food Industry, Food, and Nutrition, “Luiz de Queiroz” College of Agriculture, University of São Paulo, Av. Pádua Dias, 11, Piracicaba, SP, Brazil;Department of Physiological Sciences, Piracicaba Dental School, University of Campinas UNICAMP, Av. Limeira 901, 13414-903, Piracicaba, SP, Brazil;Research Center for Chemistry, Biology, and Agriculture, University of Campinas –UNICAMP, CP 6171, 13083-970, Campinas, SP, Brazil;
关键词: Melipona scutellaris;    Geopropolis;    Antimicrobial activity;    Antiproliferative activity;    Chemical profile;   
DOI  :  10.1186/1472-6882-13-23
 received in 2012-08-29, accepted in 2013-01-21,  发布年份 2013
来源: Springer
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【 摘 要 】

BackgroundGeopropolis is a type of propolis containing resin, wax, and soil, collected by threatened stingless bee species native to tropical countries and used in folk medicine. However, studies concerning the biological activity and chemical composition of geopropolis are scarce. In this study, we evaluated the antimicrobial and antiproliferative activity of the ethanolic extract of geopropolis (EEGP) collected by Melipona scutellaris and its bioactive fraction against important clinical microorganisms as well as their in vitro cytotoxicity and chemical profile.MethodsThe antimicrobial activity of EEGP and fractions was examined by determining their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against six bacteria strains as well as their ability to inhibit Streptococcus mutans biofilm adherence. Total growth inhibition (TGI) was chosen to assay the antiproliferative activity of EEGP and its bioactive fraction against normal and cancer cell lines. The chemical composition of M. scutellaris geopropolis was identified by reversed-phase high-performance liquid chromatography and gas chromatography–mass spectrometry.ResultsEEGP significantly inhibited the growth of Staphylococcus aureus strains and S. mutans at low concentrations, and its hexane fraction (HF) presented the highest antibacterial activity. Also, both EEGP and HF inhibited S. mutans biofilm adherence (p < 0.05) and showed selectivity against human cancer cell lines, although only HF demonstrated selectivity at low concentrations. The chemical analyses performed suggest the absence of flavonoids and the presence of benzophenones as geopropolis major compounds.ConclusionsThe empirical use of this unique type of geopropolis by folk medicine practitioners was confirmed in the present study, since it showed antimicrobial and antiproliferative potential against the cancer cell lines studied. It is possible that the major compounds found in this type of geopropolis are responsible for its properties.

【 授权许可】

Unknown   
© da Cunha et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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