Antimicrobial and antiproliferative activities of stingless bee Melipona scutellaris geopropolis

BMC Complementary and Alternative Medicine

Pedro Luiz Rosalen³  Hyun Koo²  Severino Matias de Alencar¹  Masarahu Ikegaki⁴  João Ernesto de Carvalho⁵  AnaLúciaTascaGóis de Ruiz⁵  LíviaCâmaradeCarvalho Galvão³  Marcelo Franchin³  Marcos Guilherme da Cunha³ 

[1] Department of Agri-Food Industry, Food, and Nutrition, “Luiz de Queiroz” College of Agriculture, University of São Paulo, Av. Pádua Dias, 11, Piracicaba, SP, Brazil;Center for Oral Biology, University of Rochester, 601 Elmwood Avenue, Rochester, NY, 14642, USA;Department of Physiological Sciences, Piracicaba Dental School, University of Campinas UNICAMP, Av. Limeira 901, 13414-903, Piracicaba, SP, Brazil;College of Pharmaceutical Sciences, Federal University of Alfenas, 37130–000, Alfenas, MG, Brazil;Research Center for Chemistry, Biology, and Agriculture, University of Campinas –UNICAMP, CP 6171, 13083-970, Campinas, SP, Brazil

关键词: Chemical profile;    Antiproliferative activity;    Antimicrobial activity;    Geopropolis;    Melipona scutellaris;   

Others  :  1231152
DOI  :  10.1186/1472-6882-13-23

 received in 2012-08-29, accepted in 2013-01-21,  发布年份 2013

【 摘 要 】

Background

Geopropolis is a type of propolis containing resin, wax, and soil, collected by threatened stingless bee species native to tropical countries and used in folk medicine. However, studies concerning the biological activity and chemical composition of geopropolis are scarce. In this study, we evaluated the antimicrobial and antiproliferative activity of the ethanolic extract of geopropolis (EEGP) collected by Melipona scutellaris and its bioactive fraction against important clinical microorganisms as well as their in vitro cytotoxicity and chemical profile.

Methods

The antimicrobial activity of EEGP and fractions was examined by determining their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against six bacteria strains as well as their ability to inhibit Streptococcus mutans biofilm adherence. Total growth inhibition (TGI) was chosen to assay the antiproliferative activity of EEGP and its bioactive fraction against normal and cancer cell lines. The chemical composition of M. scutellaris geopropolis was identified by reversed-phase high-performance liquid chromatography and gas chromatography–mass spectrometry.

Results

EEGP significantly inhibited the growth of Staphylococcus aureus strains and S. mutans at low concentrations, and its hexane fraction (HF) presented the highest antibacterial activity. Also, both EEGP and HF inhibited S. mutans biofilm adherence (p < 0.05) and showed selectivity against human cancer cell lines, although only HF demonstrated selectivity at low concentrations. The chemical analyses performed suggest the absence of flavonoids and the presence of benzophenones as geopropolis major compounds.

Conclusions

The empirical use of this unique type of geopropolis by folk medicine practitioners was confirmed in the present study, since it showed antimicrobial and antiproliferative potential against the cancer cell lines studied. It is possible that the major compounds found in this type of geopropolis are responsible for its properties.

【 授权许可】

   
2013 da Cunha et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Sforcin JM, Bankova V: Propolis: is there a potential for the development of new drugs? J Ethnopharmacol 2011, 133:253-260.
• [2]Silva BB, Rosalen PL, Cury JA, Ikegaki M, Souza VC, Esteves A, Alencar SM: Chemical composition and botanical origin of red propolis, a new type of Brazilian propolis. Evid Based Complement Alternat Med 2008, 5:313-316.
• [3]Dutra RP, Nogueira AMC, Marques RRO, Costa MCP, Ribeiro MNS: Avaliação farmacognóstica de geoprópolis de Melipona fasciculata Smith da Baixada maranhense. Brasil. Rev Bras Farmacogn 2008, 18:557-562.
• [4]Velikova M, Bankova V, Marcucci MC, Tsvetkova I, Kujumgiev AZ: Chemical composition and biological activity of propolis from Brazilian meliponinae. Z Naturforsch C 2000, 55:785-789.
• [5]Liberio SA, Pereira AL, Dutra RP, Reis AS, Araújo MJ, Mattar NS, Silva LA, Ribeiro MN, Nascimento FR, Guerra RN, Monteiro-Neto V: Antimicrobial activity against oral pathogens and immunomodulatory effects and toxicity of geopropolis produced by the stingless bee Melipona fasciculata Smith. BMC Complement Altern Med 2011, 11:108-117. BioMed Central Full Text
• [6]Loesche WJ: Role of Streptococcus mutans in human dental decay. Microbiol Rev 1986, 50:353-380.
• [7]Moreillon P, Que YA: Infective endocarditis. Lancet 2004, 363:139-149.
• [8]King MD, Humphrey BJ, Wang YF, Kourbatova EV, Ray SM, Blumberg HM: Emergence of community-acquired methicillin-resistant Staphylococcus aureus USA 300 clone as the predominant cause of skin and soft-tissue infections. Ann Intern Med 2006, 144:309-317.
• [9]Newman DJ, Cragg GM: Natural products as sources of new drugs over the 30 years from 1981 to 2010. J Nat Prod 2012, 75:311-335.
• [10]Umthong S, Phuwapraisirisan P, Puthong S, Chanchao C: In vitro antiproliferative activity of partially purified Trigona laeviceps propolis from Thailand on human cancer cell lines. BMC Complement Altern Med 2011, 11:37-44. BioMed Central Full Text
• [11]Franchin M, Cunha MG, Denny C, Napimoga MH, Cunha TM, Koo H, Alencar SM, Ikegaki M, Rosalen PL: Geopropolis from Melipona scutellaris decreases the mechanical inflammatory hypernociception by inhibiting the production of IL-1β and TNF-α. J Ethnopharmacol 2012, 143:709-715.
• [12]Williams ST, Goodfellow M, Wellington EM, Vickers JC, Alderson G, Sneath PH, Sackin MJ, Mortimer AM: A probability matrix for identification of some Streptomycetes. J Gen Microbiol 1983, 129:1815-1830.
• [13]Clinical and Laboratory Standards Institute: Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard. 7th edition. CLSI, Wayne, PA, USA; 2006. CLSI document M07-A7
• [14]Koo H, Rosalen PL, Cury JA, Ambrosano GM, Murata RM, Yatsuda R, Ikegaki M, Alencar SM, Park YK: Effect of a new variety of Apis mellifera propolis on mutans Streptococci. Curr Microbiol 2000, 41:192-196.
• [15]Castro ML, Nascimento AM, Ikegaki M, Costa-Neto CM, Alencar SM, Rosalen PL: Identification of a bioactive compound isolated from Brazilian propolis type 6. Bioorg Med Chem 2009, 17:5332-5335.
• [16]Galvão LC, Furletti VF, Bersan SM, Cunha MG, Ruiz AL, Carvalho JE, Sartoratto A, Rehder VL, Figueira GM, Teixeira Duarte MC, Ikegaki M, Alencar SM, Rosalen PL: Antimicrobial activity of essential oils against Streptococcus mutans and their antiproliferative effects. Evid Based Complement Alternat Med 2012 2012. Article ID 751435. http://www.hindawi.com/journals/ecam/2012/751435/ webcite
• [17]Monks A, Scudiero D, Skehan P, Shoemaker R, Paull K, Vistica D, Hose C, Langley J, Cronise P, Vaigro-Wolff A, Gray-Goodrich M, Campbell H, Mayo J, Boyd M: Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. J Natl Cancer Inst 1991, 83:757-766.
• [18]Proestos C, Sereli D, Komaitis M: Determination of phenolic compounds in aromatic plants by RP-HPLC and GC-MS. Food Chem 2006, 95:44-52.
• [19]Hayacibara MF, Koo H, Rosalen PL, Duarte S, Franco EM, Bowen WH, Ikegaki M, Cury JA: In vitro and in vivo effects of isolated fractions of Brazilian propolis on caries development. J Ethnopharmacol 2005, 101:110-115.
• [20]Duarte MC, Leme EE, Delarmelina C, Soares AA, Figueira GM, Sartoratto A: Activity of essential oils from Brazilian medicinal plants on Escherichia coli. J Ethnopharmacol 2007, 111:197-201.
• [21]Duarte S, Koo H, Bowen WH, Hayacibara MF, Cury JA, Ikegaki M, Rosalen PL: Effect of a novel type of propolis and its chemical fractions on glucosyltransferases and on growth and adherence of mutans streptococci. Biol Pharm Bull 2003, 26:527-531.
• [22]Jeon JG, Rosalen PL, Falsetta ML, Koo H: Natural products in caries research: current (limited) knowledge, challenges and future perspective. Caries Res 2011, 45:243-263.
• [23]Onlen Y, Duran N, Atik E, Savas L, Altug E, Yakan S, Aslantas O: Antibacterial activity of propolis against MRSA and synergism with topical mupirocin. J Altern Complement Med 2007, 13:713-718.
• [24]Fouche G, Cragg GM, Pillay P, Kolesnikova N, Maharaj VJ, Senabe J: In vitro anticancer screening of South African plants. J Ethnopharmacol 2008, 119:455-461.
• [25]Ishida VFC, Negri G, Salatino A, Bandeira MFCL: A new type of Brazilian propolis: Prenylated benzophenones in propolis from Amazon and effects against cariogenic bacteria. Food Chem 2011, 125:966-972.
• [26]Silva JFM, Souza MC, Matta SR, Andrade MR, Vidal FVN: Correlation analysis between phenolic levels of Brazilian propolis extracts and their antimicrobial and antioxidant activities. Food Chem 2006, 99:431-435.
• [27]Tomás-Barberán FA, García-Viguera C, Vit-Olivier P, Ferreres F, Tomás-Lorente F: Phytochemical evidence for the botanical origin of tropical propolis from Venezuela. Phytochemistry 1993, 34:191-196.
• [28]Duarte S, Rosalen PL, Hayacibara MF, Cury JA, Bowen WH, Marquis RE, Rehder VL, Sartoratto A, Ikegaki M, Koo H: The influence of a novel propolis on mutans streptococci biofilms and caries development in rats. Arch Oral Biol 2006, 51:15-22.