Genome-wide identification and evolutionary analysis of leucine-rich repeat receptor-like protein kinase genes in soybean

BMC Plant Biology

Research Article

Li-Juan Qiu¹  Fulai Zhou¹  Yong Guo¹ 

[1] The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) and MOA Key Labs of Crop Germplasm and Soybean Biology (Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences, No.12 Zhongguancun South Street, 100081, Beijing, Haidian District, P. R. China;

关键词: Soybean;    Leucine-rich repeat receptor-like kinase (LRR-RLK);    Phylogenetic analysis;    Expression profiling;    Evolutionary analysis;   

DOI  :  10.1186/s12870-016-0744-1

 received in 2015-12-06, accepted in 2016-02-24,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundLeucine-rich repeat receptor-like kinases (LRR-RLKs) constitute the largest subfamily of receptor-like kinases in plant. A number of reports have demonstrated that plant LRR-RLKs play important roles in growth, development, differentiation, and stress responses. However, no comprehensive analysis of this gene family has been carried out in legume species.ResultsBased on the principles of sequence similarity and domain conservation, a total of 467 LRR-RLK genes were identified in soybean genome. The GmLRR-RLKs are non-randomly distributed across all 20 chromosomes of soybean and about 73.3 % of them are located in segmental duplicated regions. The analysis of synonymous substitutions for putative paralogous gene pairs indicated that most of these gene pairs resulted from segmental duplications in soybean genome. Furthermore, the exon/intron organization, motif composition and arrangements were considerably conserved among members of the same groups or subgroups in the constructed phylogenetic tree. The close phylogenetic relationship between soybean LRR-RLK genes with identified Arabidopsis genes in the same group also provided insight into their putative functions. Expression profiling analysis of GmLRR-RLKs suggested that they appeared to be differentially expressed among different tissues and some of duplicated genes exhibited divergent expression patterns. In addition, artificial selected GmLRR-RLKs were also identified by comparing the SNPs between wild and cultivated soybeans and 17 genes were detected in regions previously reported to contain domestication-related QTLs.ConclusionsComprehensive and evolutionary analysis of soybean LRR-RLK gene family was performed at whole genome level. The data provides valuable tools in future efforts to identify functional divergence of this gene family and gene diversity among different genotypes in legume species.

【 授权许可】

CC BY   
© Zhou et al. 2016