L-mimosine and hypoxia can increase angiogenin production in dental pulp-derived cells

BMC Oral Health

Research Article

Michael Edelmayer¹  Andreas Moritz²  Klara Janjić²  Hermann Agis² 

[1] Austrian Cluster for Tissue Regeneration, Donaueschingenstr. 13, 1200, Vienna, Austria;Department of Oral Surgery, School of Dentistry, Medical University of Vienna, Sensengasse 2a, 1090, Vienna, Austria;Department of Conservative Dentistry and Periodontology, School of Dentistry, Medical University of Vienna, Sensengasse 2a, 1090, Vienna, Austria;Austrian Cluster for Tissue Regeneration, Donaueschingenstr. 13, 1200, Vienna, Austria;

关键词: Angiogenin;    ANG;    Dental pulp;    Prolyl hydroxylases;    Prolyl hydroxylase inhibitors;    Regeneration;    Hypoxia;    Hypoxia mimetic agents;    Microtissues;    Echinomycin;   

DOI  :  10.1186/s12903-017-0373-6

 received in 2016-12-07, accepted in 2017-05-03,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundAngiogenin is a key molecule in the healing process which has been successfully applied in the field of regenerative medicine. The role of angiogenin in dental pulp regeneration is unclear. Here we aimed to reveal the impact of the hypoxia mimetic agent L-mimosine (L-MIM) and hypoxia on angiogenin in the dental pulp.MethodsHuman dental pulp-derived cells (DPC) were cultured in monolayer and spheroid cultures and treated with L-MIM or hypoxia. In addition, tooth slice organ cultures were applied to mimic the pulp-dentin complex. We measured angiogenin mRNA and protein levels using qPCR and ELISA, respectively. Inhibitor studies with echinomycin were performed to reveal the role of hypoxia-inducible factor (HIF)-1 signaling.ResultsBoth, L-MIM and hypoxia increased the production of angiogenin at the protein level in monolayer cultures of DPC, while the increase at the mRNA level did not reach the level of significance. The increase of angiogenin in response to treatment with L-MIM or hypoxia was reduced by echinomycin. In spheroid cultures, L-MIM increased angiogenin at protein levels while the effect of hypoxia was not significant. Angiogenin was also expressed and released in tooth slice organ cultures under normoxic and hypoxic conditions and in the presence of L-MIM.ConclusionsL-MIM and hypoxia modulate production of angiogenin via HIF-1 differentially and the response depends on the culture model. Given the role of angiogenin in regeneration the here presented results are of high relevance for pre-conditioning approaches for cell therapy and tissue engineering in the field of regenerative endodontics.

【 授权许可】

CC BY   
© The Author(s). 2017