Analyses of the sucrose synthase gene family in cotton: structure, phylogeny and expression patterns

BMC Plant Biology

Research Article

Feifei Li¹  Shae He¹  Junkang Rong¹  Mingquan Ding¹  Qingpo Liu¹  Zhao Li¹  Aiqun Chen² 

[1] School of Agriculture and Food Science, Zhejiang A & F University, Lin'an, 311300, Hangzhou, Zhejiang, China;School of Agriculture and Food Science, Zhejiang A & F University, Lin'an, 311300, Hangzhou, Zhejiang, China;State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, China;

关键词: Sucrose synthase;    Gene structure;    Phylogeny;    Expression pattern;    Cotton;   

DOI  :  10.1186/1471-2229-12-85

 received in 2012-02-10, accepted in 2012-05-16,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundIn plants, sucrose synthase (Sus) is widely considered as a key enzyme involved in sucrose metabolism. Several paralogous genes encoding different isozymes of Sus have been identified and characterized in multiple plant genomes, while limited information of Sus genes is available to date for cotton.ResultsHere, we report the molecular cloning, structural organization, phylogenetic evolution and expression profiles of seven Sus genes (GaSus1 to 7) identified from diploid fiber cotton (Gossypium arboreum). Comparisons between cDNA and genomic sequences revealed that the cotton GaSus genes were interrupted by multiple introns. Comparative screening of introns in homologous genes demonstrated that the number and position of Sus introns are highly conserved among Sus genes in cotton and other more distantly related plant species. Phylogenetic analysis showed that GaSus1, GaSus2, GaSus3, GaSus4 and GaSus5 could be clustered together into a dicot Sus group, while GaSus6 and GaSus7 were separated evenly into other two groups, with members from both dicot and monocot species. Expression profiles analyses of the seven Sus genes indicated that except GaSus2, of which the transcripts was undetectable in all tissues examined, and GaSus7, which was only expressed in stem and petal, the other five paralogues were differentially expressed in a wide ranges of tissues, and showed development-dependent expression profiles in cotton fiber cells.ConclusionsThis is a comprehensive study of the Sus gene family in cotton plant. The results presented in this work provide new insights into the evolutionary conservation and sub-functional divergence of the cotton Sus gene family in response to cotton fiber growth and development.

【 授权许可】

Unknown   
© Chen et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.