Genome-wide identification and expression profile of GhGRF gene family in Gossypium hirsutum L.

PeerJ

article

Kun Liu¹  Nosheen Kabir²  Zhenzhen Wei²  Zhuojing Sun³  Jian Wang¹  Jing Qi¹  Miaoyang Liu¹  Ji Liu²  Kehai Zhou² 

[1] Henan Key Laboratory of Crop Molecular Breeding and Bioreactor, Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University;State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences;Development Center for Science and Technology, Ministry of Agriculture and Rural Affairs

关键词: Gossypium hirsutum;    Growth regulating factor;    MicroRNA396;    Gene editing;    Molecular improvement;    Collinearity;    Expression pattern;    Transcripts;    Sequence logos;    Gene structure;   

DOI  :  10.7717/peerj.13372

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundCotton is the primary source of renewable natural fiber in the textile industry and an important biodiesel crop. Growth regulating factors (GRFs) are involved in regulating plant growth and development.MethodsUsing genome-wide analysis, we identified 35 GRF genes in Gossypium hirsutum.ResultsChromosomal location information revealed an uneven distribution of GhGRF genes, with maximum genes on chromosomes A02, A05, and A12 from the At sub-genome and their corresponding D05 and D12 from the Dt sub-genome. In the phylogenetic tree, 35 GRF genes were divided into five groups, including G1, G2, G3, G4, and G5. The majority of GhGRF genes have two to three introns and three to four exons, and their deduced proteins contained conserved QLQ and WRC domains in the N-terminal end of GRFs in Arabidopsis and rice. Sequence logos revealed that GRF genes were highly conserved during the long-term evolutionary process. The CDS of the GhGRF gene can complement MiRNA396a. Moreover, most GhGRF genes transcripts developed high levels of ovules and fibers. Analyses of promoter cis-elements and expression patterns indicated that GhGRF genes play an essential role in regulating plant growth and development by coordinating the internal and external environment and multiple hormone signaling pathways. Our analysis indicated that GhGRFs are ideal target genes with significant potential for improving the molecular structure of cotton.

【 授权许可】

CC BY