Skin is the first barrier to body protection from injury and infection, one of the largest surface areas in the organs. Unlike the past, which is considered to be a simple physical barrier, recent studies have emphasized that skin is the front line organ of body homeostasis and actively contributes to the health of the body related to immunity or non-immunity. To understand the functioning of these functions, it is necessary to observe the interactions and results of each sub-group that makes up the skin. Most of the cellular functions of cells can be observed by simply modifying the genes associated with them. However, as mentioned above, the skin composed of various cell groups is a mutually important network. Therefore, it is advantageous to observe phenotypes in real-world environments through transgenic animal models as a result of interactions that may be lacking with In Vitro studies alone. In this study, we examined the immunity of skin through CD80 CD86 gene mutation and the metabolic changes through Foxn1 gene mutation. Transgenic mice were constructed using the CRISPR / Cas system, which was proved to be an efficient and relatively simple genome editing tool. The most important point in this study is that we can extract similar results as possible by analyzing phenotype considering complex and complex skin composition and function. In conclusion, this study found a phenotype of hair loss and epidermal-associated metabolic changes associated with autoimmune diseases in the skin through the transformation of a single gene.
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A Study for Phenotype Analysis with the CRISPR Mediated Mice Model on the Skin Associated Immunity and Metabolism
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