Kalanchoe tubiflora extract inhibits cell proliferation by affecting the mitotic apparatus

BMC Complementary and Alternative Medicine

Research Article

Chinpiao Chen¹  Yi-Jen Hsieh²  Ming-Yeh Yang³  Chih-Jui Chang³  Yann-Lii Leu⁴  Meng-Ya Chang⁵  Chin-Fung Wan⁶ 

[1] Department of Chemistry, National Dong-Hwa University, Hualien, Taiwan;Department of Laboratory Medicine and Biotechnology, Tzu Chi University, 97004, Hualien, Taiwan;Department of Chemistry, National Dong-Hwa University, Hualien, Taiwan;Department of Molecular Biology and Human Genetics, Tzu Chi University, No. 701, Zhongyang Rd., Sec. 3, 97004, Hualien, Taiwan;Graduate Institute of Natural Products, Chang Gung University, Taoyuan, Taiwan;Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan;Department of Medical Research, Buddhist Tzu-Chi General Hospital, Hualien, Taiwan;School of Applied Chemistry, Chung Shan Medical University, No.110,Sec.1,Jianguo N.Road, 40201, Taichung City, Taiwan;Institute of NanoEngineering and MicroSystems, National Tsing Hua University, No. 101, Section 2, Kuang-Fu Road, 30013, Hsinchu, Taiwan;

关键词: Kalanchoe tubiflora;    Multipolar spindle;    Anti-proliferation;   

DOI  :  10.1186/1472-6882-12-149

 received in 2012-03-26, accepted in 2012-08-31,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundKalanchoe tubiflora (KT) is a succulent plant native to Madagascar, and is commonly used as a medicinal agent in Southern Brazil. The underlying mechanisms of tumor suppression are largely unexplored.MethodsCell viability and wound-healing were analyzed by MTT assay and scratch assay respectively. Cell cycle profiles were analyzed by FACS. Mitotic defects were analyzed by indirect immunofluoresence images.ResultsAn n-Butanol-soluble fraction of KT (KT-NB) was able to inhibit cell proliferation. After a 48 h treatment with 6.75 μg/ml of KT, the cell viability was less than 50% of controls, and was further reduced to less than 10% at higher concentrations. KT-NB also induced an accumulation of cells in the G2/M phase of the cell cycle as well as an increased level of cells in the subG1 phase. Instead of disrupting the microtubule network of interphase cells, KT-NB reduced cell viability by inducing multipolar spindles and defects in chromosome alignment. KT-NB inhibits cell proliferation and reduces cell viability by two mechanisms that are exclusively involved with cell division: first by inducing multipolarity; second by disrupting chromosome alignment during metaphase.ConclusionKT-NB reduced cell viability by exclusively affecting formation of the proper structure of the mitotic apparatus. This is the main idea of the new generation of anti-mitotic agents. All together, KT-NB has sufficient potential to warrant further investigation as a potential new anticancer agent candidate.

【 授权许可】

Unknown   
© Hsieh et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.