Kalanchoe tubiflora extract inhibits cell proliferation by affecting the mitotic apparatus

BMC Complementary and Alternative Medicine

Chih-Jui Chang²  Meng-Ya Chang⁵  Chin-Fung Wan³  Chinpiao Chen⁴  Yann-Lii Leu¹  Ming-Yeh Yang²  Yi-Jen Hsieh⁴ 

[1] Graduate Institute of Natural Products, Chang Gung University, Taoyuan, Taiwan;Department of Molecular Biology and Human Genetics, Tzu Chi University, No. 701, Zhongyang Rd., Sec. 3, Hualien, 97004, Taiwan;Institute of NanoEngineering and MicroSystems, National Tsing Hua University, No. 101, Section 2, Kuang-Fu Road, Hsinchu, 30013, Taiwan;Department of Chemistry, National Dong-Hwa University, Hualien, Taiwan;Department of Medical Research, Buddhist Tzu-Chi General Hospital, Hualien, Taiwan

关键词: Anti-proliferation;    Multipolar spindle;    Kalanchoe tubiflora;   

Others  :  1232019
DOI  :  10.1186/1472-6882-12-149

 received in 2012-03-26, accepted in 2012-08-31,  发布年份 2012

【 摘 要 】

Background

Kalanchoe tubiflora (KT) is a succulent plant native to Madagascar, and is commonly used as a medicinal agent in Southern Brazil. The underlying mechanisms of tumor suppression are largely unexplored.

Methods

Cell viability and wound-healing were analyzed by MTT assay and scratch assay respectively. Cell cycle profiles were analyzed by FACS. Mitotic defects were analyzed by indirect immunofluoresence images.

Results

An n-Butanol-soluble fraction of KT (KT-NB) was able to inhibit cell proliferation. After a 48 h treatment with 6.75 μg/ml of KT, the cell viability was less than 50% of controls, and was further reduced to less than 10% at higher concentrations. KT-NB also induced an accumulation of cells in the G2/M phase of the cell cycle as well as an increased level of cells in the subG1 phase. Instead of disrupting the microtubule network of interphase cells, KT-NB reduced cell viability by inducing multipolar spindles and defects in chromosome alignment. KT-NB inhibits cell proliferation and reduces cell viability by two mechanisms that are exclusively involved with cell division: first by inducing multipolarity; second by disrupting chromosome alignment during metaphase.

Conclusion

KT-NB reduced cell viability by exclusively affecting formation of the proper structure of the mitotic apparatus. This is the main idea of the new generation of anti-mitotic agents. All together, KT-NB has sufficient potential to warrant further investigation as a potential new anticancer agent candidate.

【 授权许可】

   
2012 Hsieh et al.; licensee BioMed Central Ltd.

【 图 表 】

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