Local erythropoietin and endothelial progenitor cells improve regional cardiac function in acute myocardial infarction

BMC Cardiovascular Disorders

Research Article

Andreas Stein¹  Martina Knödler¹  Ilka Ott¹  Philip Groha¹  Maren Schürmann²  Eliane Weidl²  Robert AJ Oostendorp²  Sandra Kühnel²  Rene Botnar³  Stefan Nekolla³  Alexandra Keithahn³  Atti Saraste³  Markus Makowski³ 

[1] Deutsches Herzzentrum der Technischen Universität München, Lazarettstr. 36, 80636, München, Germany;Medizinische Klinik der Technischen Universität München, Ismaningerstr. 22, 81675, München, Germany;Nuklearmedizinische Klinik und Poliklinik der Technischen Universität München, Ismaningerstr. 22, 81675, München, Germany;

关键词: Infarct Size;    Regional Wall;    Border Zone;    Experimental Myocardial Infarction;    Permanent Occlusion;   

DOI  :  10.1186/1471-2261-10-43

 received in 2010-04-21, accepted in 2010-09-17,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundExpanded endothelial progenitor cells (eEPC) improve global left ventricular function in experimental myocardial infarction (MI). Erythropoietin beta (EPO) applied together with eEPC may improve regional myocardial function even further by anti-apoptotic and cardioprotective effects. Aim of this study was to evaluate intramyocardial application of eEPCs and EPO as compared to eEPCs or EPO alone in experimental MI.Methods and ResultsIn vitro experiments revealed that EPO dosed-dependently decreased eEPC and leukocyte apoptosis. Moreover, in the presence of EPO mRNA expression in eEPC of proangiogenic and proinflammatory mediators measured by TaqMan PCR was enhanced. Experimental MI was induced by ligation and reperfusion of the left anterior descending coronary artery of nude rats (n = 8-9). After myocardial transplantation of eEPC and EPO CD68+ leukocyte count and vessel density were enhanced in the border zone of the infarct area. Moreover, apoptosis of transplanted CD31 + TUNEL + eEPC was decreased as compared to transplantation of eEPCs alone. Regional wall motion of the left ventricle was measured using Magnetic Resonance Imaging. After injection of eEPC in the presence of EPO regional wall motion significantly improved as compared to injection of eEPCs or EPO alone.ConclusionIntramyocardial transplantation of eEPC in the presence of EPO during experimental MI improves regional wall motion. This was associated with an increased local inflammation, vasculogenesis and survival of the transplanted cells. Local application of EPO in addition to cell therapy may prove beneficial in myocardial remodeling.

【 授权许可】

Unknown   
© Stein et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.