Experimental assessment of antidiarrheal and antisecretory activity of 80% methanolic leaf extract of Zehneria scabra in mice

BMC Complementary and Alternative Medicine

Research Article

Abebe Ejigu Hailu¹  Wondmagegn Tamiru Tadesse¹  Abraham Fikru Mechesso²  Abyot Endale Gurmu³ 

[1] Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, Addis Ababa University, Addis Ababa, Ethiopia;School of Veterinary Medicine, Hawassa University, Hawassa, Ethiopia;Unit of Pharmacognosy, School of Pharmacy, University of Gondar, Gondar, Ethiopia;

关键词: Anti-diarrhea;    Zehneria scabra;    Anti-secretory;    Rodents;   

DOI  :  10.1186/1472-6882-14-460

 received in 2014-04-19, accepted in 2014-11-27,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe leaf of Zehneria scabra is traditionally used for the management of diarrhea in Ethiopia. Its use, however, has not been scientifically validated for safety and efficacy. The aim of this study was to investigate antidiarrheal and antisecretory effects of hydroalcolic leaf extract of Z. scabra in mice models.MethodsFor each of antidiarrheal, gastrointestinal motility and antisecretory activity study Swiss albino mice were divided in to five groups. Group I was treated as control group and received 10 ml/kg of 2% Tween-80 orally; Group II served as a positive control and took standard drug in each of the experiments orally; Group III, IV and V were test groups which received the methanolic extract orally at 100, 200 and 400 mg/kg, respectively. Depending on the model total weight of fecal output, total weight of wet feces, total number of fecal output, number of wet faeces, length of intestinal transit and intestinal weight were collected. Finally, data were analyzed using one-way ANOVA followed by Tukey’s post test.ResultIn castor oil induced diarrhea model, the extract dose produced a significant reduction in mean stool score (1.94 ± 0.102) at 200 mg/kg. Moreover, the 100, 200, and 400 mg/kg doses inhibited stool frequency by 40, 45 and 55%, respectively. All test doses of extract and loperamide (3mg/kg) reduced fecal fluid content significantly (p<0.01). The 100 mg/kg dose of extract produced 25.74% reduction of fluid content (p<0.001) while both 200 and 400 mg/kg showed 29.70 % (p<0.001) compared to negative control group.ConclusionThe extract of Zehineria scabra showed antidiarrheal and antisecretory activity in mice model. Moreover, the extract found to be safe at dose of 2000mg/kg in mice model. The findings suggest the validity of the acclaimed effect of Zehineria scabra as antidiarrheal agent in Ethiopian traditional herbal medicine.

【 授权许可】

CC BY   
© Tadesse et al.; licensee BioMed Central Ltd. 2014