BMC Complementary and Alternative Medicine | |
The ethyl acetate fraction of corn silk exhibits dual antioxidant and anti-glycation activities and protects insulin-secreting cells from glucotoxicity | |
Research Article | |
Hsiu-Chen Huang1  Hsiao-Yuh Roan2  Wei Yuan3  Jia-Ling Chang4  Hui-Kang Liu5  Huey Jen Tsay6  Chia-Chuan Chang7  Yu-Ching Lee8  | |
[1] Department of Applied Science, National Hsinchu University of Education, Hsinchu, Taiwan, ROC;Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei, Taiwan, ROC;Department of Psychiatry, Cheng Hsin General Hospital, Taipei, Taiwan, ROC;Division of Basic Chinese Medicine, National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei, Taiwan, ROC;Division of Basic Chinese Medicine, National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei, Taiwan, ROC;Ph.D Program for the Clinical Drug Discovery from Herbal Medicine, College of Pharmacy, Taipei Medical University, Taipei, Taiwan, ROC;Institute of Neuroscience, Brain Research Centre, National Yang-Ming University, Taipei, Taiwan, ROC;School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC;The Center of Translational Medicine, Taipei Medical University, Taipei, Taiwan, ROC;The Ph.D. Program for Medical Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan, ROC; | |
关键词: Stigmata Maydis (corn silk); Glucotoxicity; Methylglyoxal; Advanced glycation end products; Reactive oxygen species; β-cell failure; | |
DOI : 10.1186/s12906-016-1382-8 | |
received in 2016-05-27, accepted in 2016-10-07, 发布年份 2016 | |
来源: Springer | |
【 摘 要 】
BackgroundIn this study, we aimed to develop a Stigmata Maydis (corn silk) fraction with dual bio-activities against oxidative stress and protein glycation to protect β-cells from diabetes-induced failure.MethodsCorn silk fractions were prepared by partition and chemically characterised by thin-layer chromatography. Free radical scavenging assay, glycation assay, and cell-based viability test (neutral red) were employed to decide the best fraction. Cell death analysis was executed by annexin V/ Propidium iodide staining. Cell proliferation was measured by WST-1. Finally, β-cell function was evaluated by β-cell marker gene expression (RT-PCR) and acute insulin secretion test.ResultsFour corn silk fractions were prepared from an ethanolic crude extract of corn silk. In vitro assays indicate ethyl acetate fraction (YMS-EA) was the most potent fraction. YMS-EA also attenuated the hydrogen peroxide- or methylglyoxal-induced induction of reactive oxygen species, reduction of cell viability, and inhibition of cell proliferation. However, YMS-EA was unable to prevent hydrogen peroxide-induced apoptosis or advanced glycation end-products-induced toxicity. Under hyperglycemic conditions, YMS-EA effectively reduced ROS levels, improved mRNA expression of insulin, glucokinase, and PDX-1, and enhanced glucose-stimulated insulin secretion. The similarity of bioactivities among apigenin, luteolin, and YMS-EA indicated that dual activities of YMS-EA might be derived from those compounds.ConclusionsWe concluded that YMS-EA fraction could be developed as a preventive food agent against the glucotoxicity to β-cells in Type 2 diabetes.
【 授权许可】
CC BY
© The Author(s). 2016
【 预 览 】
Files | Size | Format | View |
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RO202311091110569ZK.pdf | 2082KB | download |
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