Mechano-stimulated modifications in the chloroplast antioxidant system and proteome changes are associated with cold response in wheat

BMC Plant Biology

Research Article

Fulai Liu¹  Xiao Wang²  Jianwen Zhong²  Weixing Cao²  Jian Cai²  Chenglong Hao²  Dong Jiang²  Qin Zhou²  Tingbo Dai²  Xiangnan Li³ 

[1] Faculty of Science, Department of Plant and Environmental Sciences, University of Copenhagen, Højbakkegaard Allé 13, DK-2630, Taastrup, Denmark;National Engineering and Technology Center for Information Agriculture / Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture, Nanjing Agricultural University, 210095, Nanjing, China;National Engineering and Technology Center for Information Agriculture / Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture, Nanjing Agricultural University, 210095, Nanjing, China;Faculty of Science, Department of Plant and Environmental Sciences, University of Copenhagen, Højbakkegaard Allé 13, DK-2630, Taastrup, Denmark;

关键词: Mechano-stimulation;    Cold;    Reactive oxygen species;    Chloroplast;    Wheat;   

DOI  :  10.1186/s12870-015-0610-6

 received in 2015-06-24, accepted in 2015-09-09,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundMechanical wounding can cause morphological and developmental changes in plants, which may affect the responses to abiotic stresses. However, the mechano-stimulation triggered regulation network remains elusive. Here, the mechano-stimulation was applied at two different times during the growth period of wheat before exposing the plants to cold stress (5.6 °C lower temperature than the ambient temperature, viz., 5.0 °C) at the jointing stage.ResultsResults showed that mechano-stimulation at the Zadoks growth stage 26 activated the antioxidant system, and substantially, maintained the homeostasis of reactive oxygen species. In turn, the stimulation improved the electron transport and photosynthetic rate of wheat plants exposed to cold stress at the jointing stage. Proteomic and transcriptional analyses revealed that the oxidative stress defense, ATP synthesis, and photosynthesis-related proteins and genes were similarly modulated by mechano-stimulation and the cold stress.ConclusionsIt was concluded that mechano-stimulated modifications of the chloroplast antioxidant system and proteome changes are related to cold tolerance in wheat. The findings might provide deeper insights into roles of reactive oxygen species in mechano-stimulated cold tolerance of photosynthetic apparatus, and be helpful to explore novel approaches to mitigate the impacts of low temperature occurring at critical developmental stages.

【 授权许可】

CC BY   
© Li et al. 2015