Membrane-enriched proteome changes and prion protein expression during neural differentiation and in neuroblastoma cells

BMC Genomics

Research Article

E. P. Melo¹  I. Duarte¹  E. Tavares¹  J. A. Macedo¹  P. M. Rodrigues²  D. Schrama²  M. E. Futschik³  J. Renaut⁴ 

[1] CBMR, Center for Biomedical Research, University of Algarve, Campus de Gambelas, Faro, Portugal;CCMAR, Centre of Marine Sciences of Algarve, University of Algarve, Campus de Gambelas, Faro, Portugal;CCMAR, Centre of Marine Sciences of Algarve, University of Algarve, Campus de Gambelas, Faro, Portugal;School of Biomedical & Healthcare Sciences, Plymouth University Peninsula Schools of Medicine and Dentistry, Plymouth, UK;LIST, Luxembourg Institute of Science and Technology, Belvaux, Luxembourg;

关键词: Prion protein;    2D-DIGE;    Neural differentiation;    Chaperone activity;    Redox homeostasis;   

DOI  :  10.1186/s12864-017-3694-6

 received in 2016-11-18, accepted in 2017-04-08,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe function of the prion protein, involved in the so-called prion diseases, remains a subject of intense debate and the possibility that it works as a pleiotropic protein through the interaction with multiple membrane proteins is somehow supported by recent reports. Therefore, the use of proteomic and bioinformatics combined to uncover cellular processes occurring together with changes in the expression of the prion protein may provide further insight into the putative pleiotropic role of the prion protein.ResultsThis study assessed the membrane-enriched proteome changes accompanying alterations in the expression of the prion protein. A 2D-DIGE approach was applied to two cell lines after prefractionation towards the membrane protein subset: an embryonic stem cell line and the PK1 subline of neuroblastoma cells which efficiently propagates prion infection. Several proteins were differentially abundant with the increased expression of the prion protein during neural differentiation of embryonic stem cells and with the knockdown of the prion protein in PK1 cells. The identity of around 20% of the differentially abundant proteins was obtained by tandem MS. The catalytic subunit A of succinate dehydrogenase, a key enzyme for the aerobic energy metabolism and redox homeostasis, showed a similar abundance trend as the prion protein in both proteomic experiments. A gene ontology analysis revealed “myelin sheath”, “organelle membrane” and “focal adhesion” associated proteins as the main cellular components, and “protein folding” and “ATPase activity” as the biological processes enriched in the first set of differentially abundant proteins. The known interactome of these differentially abundant proteins was customized to reveal four interactors with the prion protein, including two heat shock proteins and a protein disulfide isomerase.ConclusionsOverall, our study shows that expression of the prion protein occurs concomitantly with changes in chaperone activity and cell-redox homeostasis, emphasizing the functional link between these cellular processes and the prion protein.

【 授权许可】

CC BY   
© The Author(s). 2017