Blockade of nicotine sensitization by methanol extracts of Glycyrrhizae radix mediated via antagonism of accumbal oxidative stress

BMC Complementary and Alternative Medicine

Research Article

Sang Chan Kim¹  Bong Hyeo Lee¹  Chul Won Lee¹  Il Je Cho¹  Chae Ha Yang¹  Hong Feng Liu²  Yi Yan Wu²  Yu Hua Wang³  Li Bo Li³  Yu Jiao³  Zheng Lin Zhao³  Rong Jie Zhao⁴  Yu Fan⁴ 

[1] College of Korean Medicine, Daegu Haany University, 38610, Gyeongsan, Republic of Korea;Department of Pharmacology, Mudanjiang Medical University, 157011, Mudanjiang, China;School of Mental Health, Qiqihar Medical University, 333 Bukuibei Street, Jianhua District, 161006, Qiqihar, China;School of Mental Health, Qiqihar Medical University, 333 Bukuibei Street, Jianhua District, 161006, Qiqihar, China;College of Korean Medicine, Daegu Haany University, 38610, Gyeongsan, Republic of Korea;

关键词: Nicotine;    Sensitization;    Glycyrrhizae;    Dopamine;    Nucleus accumbens;    Oxidative stress;   

DOI  :  10.1186/s12906-017-1999-2

 received in 2017-07-21, accepted in 2017-11-10,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundWe previously reported that a methanol extract of Glycyrrhizae radix (MEGR) blocked methamphetamine-induced locomotor sensitization and conditioned place preference in rats. In the present study, the effects of MEGR on repeated nicotine-induced locomotor sensitization and enhanced extracellular dopamine (DA) release in the nucleus accumbens (Nacc) were evaluated.MethodsMale Sprague–Dawley rats received repeated administrations of nicotine (0.4 mg/kg, subcutaneous) or saline twice a day for 7 d and were challenged with nicotine 4 d after the last daily dosing. During the 4-d withdrawal period, the rats were treated once a day with MEGR (60 or 180 mg/kg/d). Extracellular DA levels were measured by in vivo microdialysis, the malondialdehyde levels and the activities of superoxide dismutase and catalase in the Nacc were biochemically evaluated, and the expression of antioxidant proteins was confirmed by Western blot assays. All data were assessed with analysis of variance tests followed by post-hoc comparison tests and p values <0.05 were considered statistically significant.ResultsThe expression of repeated nicotine-induced locomotor sensitization was dose-dependently attenuated by MEGR, and 180 mg/kg/d MEGR significantly inhibited augmented accumbal DA release induced by a direct local challenge of nicotine. Moreover, 180 mg/kg/d MEGR reversed increases in malondialdehyde production, decreases in superoxide dismutase and catalase activities, and the reduced expression of nuclear factor erythroid 2–related factor 2 and heme oxygenase 1 in the nicotine-sensitized Nacc.ConclusionsThese results suggest that MEGR inhibited nicotine-induced locomotion and dopaminergic sensitization via antioxidant action.

【 授权许可】

CC BY   
© The Author(s). 2017