Complete genome sequence of Saccharothrix espanaensis DSM 44229T and comparison to the other completely sequenced Pseudonocardiaceae

BMC Genomics

Research Article

Jochen Blom¹  Andreas Bechthold²  Marta Luzhetska²  Tina Strobel²  Arne Gessner²  Alfred Pühler³  Jörn Kalinowski⁴  Rafael Szczepanowski⁴  Arwa Al-Dilaimi⁴  Christian Rückert⁴ 

[1] Bioinformatics Resource Facility, CeBiTec, Bielefeld University, 33615, Bielefeld, Germany;Department of Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, Albert-Ludwigs-University, 79104, Freiburg, Germany;Senior Research Group in Genome Research of Industrial Microorganisms, CeBiTec, Bielefeld University, 33615, Bielefeld, Germany;Technology Platform Genomics, CeBiTec, Bielefeld University, 33615, Bielefeld, Germany;

关键词: Taurine;    Core Genome;    Biosynthetic Gene Cluster;    Accessory Gene;    Nonribosomal Peptide;   

DOI  :  10.1186/1471-2164-13-465

 received in 2012-04-27, accepted in 2012-08-30,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThe genus Saccharothrix is a representative of the family Pseudonocardiaceae, known to include producer strains of a wide variety of potent antibiotics. Saccharothrix espanaensis produces both saccharomicins A and B of the promising new class of heptadecaglycoside antibiotics, active against both bacteria and yeast.ResultsTo better assess its capabilities, the complete genome sequence of S. espanaensis was established. With a size of 9,360,653 bp, coding for 8,501 genes, it stands alongside other Pseudonocardiaceae with large genomes. Besides a predicted core genome of 810 genes shared in the family, S. espanaensis has a large number of accessory genes: 2,967 singletons when compared to the family, of which 1,292 have no clear orthologs in the RefSeq database. The genome analysis revealed the presence of 26 biosynthetic gene clusters potentially encoding secondary metabolites. Among them, the cluster coding for the saccharomicins could be identified.ConclusionS. espanaensis is the first completely sequenced species of the genus Saccharothrix. The genome discloses the cluster responsible for the biosynthesis of the saccharomicins, the largest oligosaccharide antibiotic currently identified. Moreover, the genome revealed 25 additional putative secondary metabolite gene clusters further suggesting the strain’s potential for natural product synthesis.

【 授权许可】

Unknown   
© Strobel et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.