Microbiome and metagenomic analysis of Lake Hillier Australia reveals pigment-rich polyextremophiles and wide-ranging metabolic adaptations

Environmental Microbiome

Research

Scott W. Tighe¹  Jordan Ramsdell²  Nathan J. Bivens³  W. Kelley Thomas⁴  Daniel Butler⁵  Stefan J. Green⁶  Ken McGrath⁷  Braden T. Tierney⁸  Jonathan Foox⁸  Krista A. Ryon⁸  Evan Afshin⁸  Christopher E. Mason⁹  Maria A. Sierra^1,10  Chandrima Bhattacharya^1,10 

[1] Advanced Genomics Laboratory, University of Vermont Cancer Center, University of Vermont, Burlington, VT, USA;BioTeam, Inc., Middleton, MA, USA;DNA Core Facility, University of Missouri, Columbia, MO, USA;Department of Molecular, Cellular, and Biomedical Sciences, College of Life Sciences and Agriculture, University of New Hampshire, Durham, NH, USA;Department of Physiology and Biophysics, Weill Cornell Medicine, 10065, New York, NY, USA;Genomics and Microbiome Core Facility, Rush University, New York, IL, USA;Microba, Brisbane City, QLD, Australia;The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, 10065, New York, NY, USA;Department of Physiology and Biophysics, Weill Cornell Medicine, 10065, New York, NY, USA;The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, 10065, New York, NY, USA;Department of Physiology and Biophysics, Weill Cornell Medicine, 10065, New York, NY, USA;WorldQuant Initiative for Quantitative Prediction, Weill Cornell Medicine, New York, NY, USA;The Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY, USA;Tri-Institutional Computational Biology and Medicine Program, Weill Cornell Medicine, New York, NY, USA;The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, 10065, New York, NY, USA;

关键词: Hillier Lake;    Hypersaline lake;    Polyextremophile;    Pigments;    Microbiome;    Metagenomics;    Biosynthetic Gene Cluster;   

DOI  :  10.1186/s40793-022-00455-9

 received in 2022-02-25, accepted in 2022-12-01,  发布年份 2022

来源: Springer

【 摘 要 】

Lake Hillier is a hypersaline lake known for its distinctive bright pink color. The cause of this phenomenon in other hypersaline sites has been attributed to halophiles, Dunaliella, and Salinibacter, however, a systematic analysis of the microbial communities, their functional features, and the prevalence of pigment-producing-metabolisms has not been previously studied. Through metagenomic sequencing and culture-based approaches, our results evidence that Lake Hillier is composed of a diverse set of microorganisms including archaea, bacteria, algae, and viruses. Our data indicate that the microbiome in Lake Hillier is composed of multiple pigment-producer microbes, including Dunaliella, Salinibacter, Halobacillus, Psychroflexus, Halorubrum, many of which are cataloged as polyextremophiles. Additionally, we estimated the diversity of metabolic pathways in the lake and determined that many of these are related to pigment production. We reconstructed complete or partial genomes for 21 discrete bacteria (N = 14) and archaea (N = 7), only 2 of which could be taxonomically annotated to previously observed species. Our findings provide the first metagenomic study to decipher the source of the pink color of Australia’s Lake Hillier. The study of this pink hypersaline environment is evidence of a microbial consortium of pigment producers, a repertoire of polyextremophiles, a core microbiome and potentially novel species.

【 授权许可】

CC BY   
© The Author(s) 2022

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