Analysis of the duodenal microbiotas of weaned piglet fed with epidermal growth factor-expressed Saccharomyces cerevisiae

BMC Microbiology

Research Article

Zhongwei Zhang¹  Yan Zhou¹  Shujin Wang²  Lili Cao³  Lin Zhou⁴ 

[1]Department of Intensive Care Unit, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan, People’s Republic of China

[2]Human and Animal Physiology, Wageningen University, 6700 AH, Wageningen, The Netherlands

[3]Medical School, Chengdu University, 610041, Chengdu, Sichuan, People’s Republic of China

[4]Shenzhen Premix Inve Nutrition Co., LTD, 518103, Shenzhen, People’s Republic of China

关键词: Bacterial community;    Full-length 16S rRNA;    Saccharomyces cerevisiae;    Epidermal growth factor;    Weaned piglet;   

DOI  :  10.1186/s12866-016-0783-7

 received in 2016-04-02, accepted in 2016-07-15,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe bacterial community of the small intestine is a key factor that has strong influence on the health of gastrointestinal tract (GIT) in mammals during and shortly after weaning. The aim of this study was to analyze the effects of the diets of supplemented with epidermal growth factor (EGF)-expressed Saccharomyces cerevisiae (S. cerevisiae) on the duodenal microbiotas of weaned piglets.ResultsRevealed in this study, at day 7, 14 and 21, respectively, the compositional sequencing analysis of the 16S rRNA in the duodenum had no marked difference in microbial diversity from the phylum to species levels between the INVSc1(EV) and other recombinant strains encompassing INVSc1-EE(+), INVSc1-TE(−), and INVSc1-IE(+). Furthermore, the populations of potentially enterobacteria (e.g., Clostridium and Prevotella) and probiotic (e.g., Lactobacilli and Lactococcus) also remained unchanged among recombinant S. cerevisiae groups (P > 0.05). However, the compositional sequencing analysis of the 16S rRNA in the duodenum revealed significant difference in microbial diversity from phylum to species levels between the control group and recombinant S. cerevisiae groups. In terms of the control group (the lack of S. cerevisiae), these data confirmed that dietary exogenous S. cerevisiae had the feasibility to be used as a supplement for enhancing potentially probiotic (e.g., Lactobacilli and Lactococcus) (P < 0.01), and reducing potentially pathogenic bacteria (e.g., Clostridium and Prevotella) (P < 0.01).ConclusionHerein, altered the microbiome effect was really S. cerevisiae, and then different forms of recombinant EGF, including T-EGF, EE-EGF and IE-EGF, did not appear to make a significant difference to the microbiome of weaned piglets.

【 授权许可】

CC BY   
© The Author(s). 2016