Pyrosequencing reveals benthic bacteria changes responsing to heavy deposition of Microcystis scum in lab — searching bacteria for bloom control

International Symposium on Resource Exploration and Environmental Science

地球科学;生态环境科学

Tang, Yali^1 ; Cheng, Dongmei^1 ; Guan, Baohua^2 ; Zhang, Xiufeng^1 ; Liu, Zhengwen^1,2 ; Liu, Zejun^1

Department of Ecology, Institute of Hydrobiology, Jinan University, China^1

State Key Laboratory of Lake and Environment, Nanjing Institute of Geography and Limnology, China^2

关键词: Bacterial community;    Bacterial community composition;    Bacterial species;    Ecological consequences;    Experimental system;    Hierarchical cluster trees;    Microcystis blooms;    Vertical gradients;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/64/1/012116/pdf
DOI  :  10.1088/1755-1315/64/1/012116

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Bacteria capable of degrading cyanobacteria Microcystis are crucial for determining the ecological consequences of Microcystis blooms in freshwater lakes. Scum derived from Microcystis blooms tends to accumulate in bays of large lakes and then sink to the sediments where it is finally consumed by benthic bacteria. Understanding the response of benthic bacterial communities to massive Microcystis deposition events may help identify the bacteria best suited to Microcystis hydrolyzation and even bloom control. For that purpose, an experimental system was set up in which intact sediment cores were incubated in the laboratory with normal and heavy deposits of Microcystis detritus. Pyrosequencing was performed in order to describe a phylogenetic inventory of bacterial communities in samples taken at 0-1, 1-2 and 2-3 cm depths in incubated sediments and in original untreated sediment. A hierarchical cluster tree was constructed expose differences between sediments. Similarity percentage calculations were also performed to identify the bacterial species contributing to variation. The results of this study suggest that: (1) deposition of Microcystis scums exerts a strong effect on the bacterial community composition in the surface (0-1 cm) and sub-surface (1-2 cm) sediment layers; (2) bacterial community responses to Microcystis detritus deposition vary across vertical gradients. A list of bacteria with potential roles in Microcystis degradation was compiled. These findings may inform the development of future measures for Microcystis bloom control in lakes.