Experimental research on the relationship between the stiffness and the expressions of fibronectin proteins and adaptor proteins of rat trabecular meshwork cells

BMC Ophthalmology

Research Article

Zhicheng Liu¹  Chuan Wang²  Lin Li³ 

[1] Department of Biomechanics and Rehabilitation Engineering, School of Biomedical Engineering, Capital Medical University, No.10 Xitoutiao, You An Men, 100069, Beijing, People’s Republic of China;Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application, Capital Medical University, 100069, Beijing, China;Department of Biomechanics and Rehabilitation Engineering, School of Biomedical Engineering, Capital Medical University, No.10 Xitoutiao, You An Men, 100069, Beijing, People’s Republic of China;Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application, Capital Medical University, 100069, Beijing, China;YanJing Medical College, Capital Medical University, 100069, Beijing, China;Department of Biomedical Informatics, School of Biomedical Engineering, Capital Medical University, No.10 Xitoutiao, You An Men, 100069, Beijing, People’s Republic of China;

关键词: TM cells stiffness;    Intraocular pressure;    AFM;    Outflow resistance;    Protein expression;   

DOI  :  10.1186/s12886-017-0662-5

 received in 2017-05-26, accepted in 2017-12-12,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundTrabecular meshwork (TM) plays an important role in maintaining normal intraocular pressure (IOP). Studies have shown that glaucomatous TM tissues are stiffer than those of normal tissue. The high expression of fibronectin protein (FN) and adaptor protein (LNK) may be related to high resistance to aqueous humor outflow as well as high IOP. Our concern is what factors lead to the variation of the stiffness of trabecular tissue/cells.MethodsAtomic force microscope (AFM) and Western blot (WB) analysis were applied to test TM cells of rats cultured with different concentrations of dexamethasone (DEX) and mifepristone (MIF). Rat TM cells were randomly divided into 7 groups, marked as D1, D2, D3 and M1, M2 M3 for different concentrations of DEX and MIF, respectively, and C for blank control.ResultsThe elastic modulus of the treated cells were 2.67 ± 0.914 KPa, 2.92 ± 0.986 KPa, 4.52 ± 1.22 KPa for D1, D2, D3, 2.06 ± 0.745 KPa, 1.23 ± 0.462 KPa, 0.467 ± 0.275 KPa for M1, M2, M3, and 2.43 ± 0.713 KPa for C group, respectively. Expressions of FN and LNK increase (decrease) with the increase of the concentrations of DEX (MIF).DiscussionWe focus on the relationship between the stiffness and the expressions of FN and LNK of rat TM cells. We analyzed the correlation between cell stiffness and FN, LNK expression, discussed the relationship between cell stiffness and aqueous humor outflow resistance.ConclusionsThe changes of TM cell stiffness and the expressions of FN and LNK are positively correlated.

【 授权许可】

CC BY   
© The Author(s). 2017