Effects of vitamin E supplementation on renal non-enzymatic antioxidants in young rats submitted to exhaustive exercise stress

BMC Complementary and Alternative Medicine

Research Article

Luiz Carlos de Abreu¹  Helio Vannucchi¹  Vitor E Valenti²  Claudio Leone³  Sérvio A Bucioli⁴ 

[1] Departamento de Morfologia e Fisiologia, Faculdade de Medicina do ABC, Santo André, SP, Brazil;Departamento de Morfologia e Fisiologia, Faculdade de Medicina do ABC, Santo André, SP, Brazil;Departamento de Fonoaudiologia, Faculdade de Filosofia e Ciências, Universidade Estadual Paulista, UNESP, Marília, SP, Brazil;Departamento de Saúde Materno-infantil, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, Brazil;Laboratório de Química e Bioquímica de Alimentos, Universidade de São Paulo (USP), São Paulo, SP, Brazil;

关键词: Thiobarbituric Acid Reactive Substance;    Exercise Stress;    Exhaustive Exercise;    Chronic Kidney Failure;    Renal Oxidative Stress;   

DOI  :  10.1186/1472-6882-11-133

 received in 2011-08-04, accepted in 2011-12-20,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundExercise stress was shown to increase oxidative stress in rats. It lacks reports of increased protection afforded by dietary antioxidant supplements against ROS production during exercise stress. We evaluated the effects of vitamin E supplementation on renal non-enzymatic antioxidants in young rats submitted to exhaustive exercise stress.MethodsWistar rats were divided into three groups: 1) control group; 2) exercise stress group and; 3) exercise stress + Vitamin E group. Rats from the group 3 were treated with gavage administration of 1 mL of Vitamin E (5 mg/kg) for seven consecutive days. Animals from groups 2 and 3 were submitted to a bout of swimming exhaustive exercise stress. Kidney samples were analyzed for Thiobarbituric Acid Reactive Substances to (TBARS) by malondialdehyde (MDA), reduced glutathione (GSH) and vitamin-E levels.ResultsThe group treated with vitamin E and submitted to exercise stress presented the lowest levels of renal MDA (1: 0.16+0.02 mmmol/mgprot vs. 2: 0.34+0.07 mmmol/mgprot vs. 3: 0.1+0.01 mmmol/mgprot; p < 0.0001), the highest levels of renal GSH (1: 23+4 μmol/gprot vs. 2: 23+2 μmol/gprot vs. 3: 58+9 μmol/gprot; p < 0.0001) and the highest levels of renal vitamin E (1: 24+6 μM/gtissue vs. 2: 28+2 μM/gtissue vs. 3: 43+4 μM/gtissue; p < 0.001).ConclusionVitamin E supplementation improved non-enzymatic antioxidant activity in young rats submitted to exhaustive exercise stress.

【 授权许可】

CC BY   
© Bucioli et al; licensee BioMed Central Ltd. 2011