Bacteriocin-encoding genes and ExPEC virulence determinants are associated in human fecal Escherichia coli strains

BMC Microbiology

Research Article

Lenka Micenková¹  Barbora Štaudová¹  Lenka Mikalová¹  David Šmajs¹  Juraj Bosák¹  Alena Ševčíková²  Martin Vrba²  Vladana Woznicová³  Simona Littnerová⁴ 

[1] Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 625 00, Brno, Czech Republic;Department of Clinical Microbiology, University Hospital Brno, Jihlavská 20, 625 00, Brno, Czech Republic;Department of Microbiology, Faculty of Medicine, Masaryk University and St. Anne's University Hospital, Pekařská 53, 656 91, Brno, Czech Republic;Institute of Biostatistics and Analyses, Masaryk University, Kamenice 3, Building A1, 625 00, Brno, Czech Republic;

关键词: Escherichia coli;    Colicin;    Microcin;    Bacteriocin;    Virulence factor;   

DOI  :  10.1186/1471-2180-14-109

 received in 2013-12-17, accepted in 2014-04-22,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundA set of 1181 E. coli strains of human fecal origin isolated in the South Moravia region of the Czech Republic was collected during the years 2007–2010. Altogether, 17 virulence determinants and 31 bacteriocin-encoding genes were tested in each of them.ResultsThe occurrence of bacteriocin-encoding genes was found to be positively correlated with the occurrence of E. coli virulence factors. Based on the presence of virulence factors and their combinations, E. coli strains were classified as non-pathogenic E. coli (n = 399), diarrhea-associated E. coli (n = 179) and ExPEC strains (n = 603). Non-pathogenic and diarrhea-associated E. coli strains had a low frequency of bacteriocinogeny (32.6% and 36.9%, respectively). ExPEC strains encoding S-fimbriae (sfa), P-fimbriae (pap) and having genes for aerobactin biosynthesis (aer, iucC), α-hemolysis (α-hly) and cytotoxic necrosis factor (cnf1) were often bacteriocinogenic (73.8%), had a high prevalence of bacteriocin multi-producers and showed a higher frequency of genes encoding microcins H47, M, V, B17 and colicins E1, Ia and S4.ConclusionsThe occurrence of bacteriocin-encoding genes and ExPEC virulence determinants correlate positively in E. coli strains of human fecal origin. Bacteriocin synthesis appears to modulate the ability of E. coli strains to reside in the human intestine and/or the virulence of the corresponding strains.

【 授权许可】

Unknown   
© Micenková et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.